BMRB Entry 15514

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15514

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Title:
Assignment of backbone 1H, 13C and 15N resonances of human IgG1 Fc (51.4 kDa)
Deposition date:
2007-10-09
Original release date:
2008-01-28
Authors:
Cocco, Melanie; Rosenfied, Robert; Lewis, Jeff; Ren, Da; Li, Luke; Remmele, Richard; Brems, David
Citation:

Citation: Liu, Dingjiang; Cocco, Melanie; Rosenfied, Robert; Lewis, Jeffery; Ren, Da; Li, Luke; Remmele, Richard; Brems, David. "Assignment of backbone (1)H, (13)C and (15)N resonances of human IgG1 Fc (51.4 kDa)"  Biomol. NMR Assignments 1, 233-235 (2007).
PubMed: 19636873

Assembly members:

Assembly members:
IgG1_Fc, polymer, 228 residues, 25679 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pAM

Entity Sequences (FASTA):

Entity Sequences (FASTA):
IgG1_Fc: MDKTHTCPPCPAPELLGGPS VFLFPPKPKDTLMISRTPEV TCVVVDVSHEDPEVKFNWYV DGVEVHNAKTKPREEQYNST YRVVSVLTVLHQDWLNGKEY KCKVSNKALPAPIEKTISKA KGQPREPQVYTLPPSRDELT KNQVSLTCLVKGFYPSDIAV EWESNGQPENNYKTTPPVLD SDGSFFLYSKLTVDKSRWQQ GNVFSCSVMHEALHNHYTQK SLSLSPGK

Data sets:
Data typeCount
13C chemical shifts573
15N chemical shifts195
1H chemical shifts195

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1human IgG1 Fc, chain 11
2human IgG1 Fc, chain 21

Entities:

Entity 1, human IgG1 Fc, chain 1 228 residues - 25679 Da.

1   METASPLYSTHRHISTHRCYSPROPROCYS
2   PROALAPROGLULEULEUGLYGLYPROSER
3   VALPHELEUPHEPROPROLYSPROLYSASP
4   THRLEUMETILESERARGTHRPROGLUVAL
5   THRCYSVALVALVALASPVALSERHISGLU
6   ASPPROGLUVALLYSPHEASNTRPTYRVAL
7   ASPGLYVALGLUVALHISASNALALYSTHR
8   LYSPROARGGLUGLUGLNTYRASNSERTHR
9   TYRARGVALVALSERVALLEUTHRVALLEU
10   HISGLNASPTRPLEUASNGLYLYSGLUTYR
11   LYSCYSLYSVALSERASNLYSALALEUPRO
12   ALAPROILEGLULYSTHRILESERLYSALA
13   LYSGLYGLNPROARGGLUPROGLNVALTYR
14   THRLEUPROPROSERARGASPGLULEUTHR
15   LYSASNGLNVALSERLEUTHRCYSLEUVAL
16   LYSGLYPHETYRPROSERASPILEALAVAL
17   GLUTRPGLUSERASNGLYGLNPROGLUASN
18   ASNTYRLYSTHRTHRPROPROVALLEUASP
19   SERASPGLYSERPHEPHELEUTYRSERLYS
20   LEUTHRVALASPLYSSERARGTRPGLNGLN
21   GLYASNVALPHESERCYSSERVALMETHIS
22   GLUALALEUHISASNHISTYRTHRGLNLYS
23   SERLEUSERLEUSERPROGLYLYS

Samples:

sample_1: IgG1 Fc, [U-13C; U-15N; U-2H], 2.5 mM; sodium acetate 10 mM

sample_conditions_1: ionic strength: 0.2 M; pH: 5.0; pressure: 1 atm; temperature: 302 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - data analysis

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

BMRB 25224
PDB
DBJ BAC86226
EMBL CAA49866 CAE45920 CAM91874 CAP05188 CAP05190
GB AAB37424 AAD38158 AAF42993 AAG00448 AAG00449
PIR PT0207 S69339
PRF 1605217A

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks