BMRB Entry 15508
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15508
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Citation: Jones, Dafydd; Stott, Katherine; Howard, Mark; Perham, Richard. "Restricted motion of the lipoyl-lysine swinging arm in the pyruvate dehydrogenase complex of Escherichia coli" Biochemistry 39, 8448-8459 (2000).
PubMed: 10913250
Assembly members:
lipoyl_domain, polymer, 79 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET11c
Entity Sequences (FASTA):
lipoyl_domain: VKEVNVPDIGGDEVEVTEVM
VKVGDKVAAEQSLITVEGDK
ASMEVPAPFAGVVKELKVNV
GDKVKTGSLIMIFEVEGAA
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 76 |
| 1H chemical shifts | 285 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | E2plip | 1 |
Entities:
Entity 1, E2plip 79 residues - Formula weight is not available
| 1 | VAL | LYS | GLU | VAL | ASN | VAL | PRO | ASP | ILE | GLY | ||||
| 2 | GLY | ASP | GLU | VAL | GLU | VAL | THR | GLU | VAL | MET | ||||
| 3 | VAL | LYS | VAL | GLY | ASP | LYS | VAL | ALA | ALA | GLU | ||||
| 4 | GLN | SER | LEU | ILE | THR | VAL | GLU | GLY | ASP | LYS | ||||
| 5 | ALA | SER | MET | GLU | VAL | PRO | ALA | PRO | PHE | ALA | ||||
| 6 | GLY | VAL | VAL | LYS | GLU | LEU | LYS | VAL | ASN | VAL | ||||
| 7 | GLY | ASP | LYS | VAL | LYS | THR | GLY | SER | LEU | ILE | ||||
| 8 | MET | ILE | PHE | GLU | VAL | GLU | GLY | ALA | ALA |
Samples:
sample_1: lipoyl domain, [U-100% 15N], 2-3 mM
sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
ANSIG, Kraulis - chemical shift assignment
NMR spectrometers:
- Bruker AM 500 MHz
Related Database Links:
| PDB | |
| EMBL | CSG13687 CSG32591 CSG39424 |
| GB | EFI22553 EFZ46147 EGB41640 EGB60459 EGI12521 |
| REF | WP_000295776 WP_000374927 WP_032230881 WP_050541395 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks