BMRB Entry 15499

Name: Solution Structure of human NEMO zinc finger
Published: 2008-03-13

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PDB ID: 2jvx
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15499
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of human NEMO zinc finger

Deposition date:

2007-09-28

Original release date:

2008-03-13

Authors:

Cordier, Florence; Vinolo, Emilie; Veron, Michel; Delepierre, Muriel; Agou, Fabrice

Citation:

Citation: Cordier, Florence; Vinolo, Emilie; Veron, Michel; Delepierre, Muriel; Agou, Fabrice. "Solution Structure of NEMO Zinc Finger and Impact of an Anhidrotic Ectodermal Dysplasia with Immunodeficiency-related Point Mutation" J. Mol. Biol. 377, 1419-1432 (2008).
PubMed: 18313693

Assembly members:

Assembly members:
ZN, non-polymer, 65.409 Da.
NEMO_ZF, polymer, 28 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NEMO_ZF: SSDFCCPKCQYQAPDMDTLQ IHVMECIE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	178

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ZINC ION	1
2	NEMO ZF	2

Entities:

Entity 1, ZINC ION - Zn - 65.409 Da.

1

ZN

Entity 2, NEMO ZF 28 residues - Formula weight is not available

Residues 3-28 corresponds to residues 394-419 of human NEMO zinc finger. Two Pro in the original sequence are replaced by two Ser (residues 1-2)

1

SER

ASP

PHE

CYS

PRO

LYS

CYS

GLN

2

TYR

GLN

ALA

PRO

ASP

MET

ASP

THR

LEU

GLN

3

ILE

HIS

VAL

MET

GLU

CYS

ILE

GLU

Samples:

sample_1: NEMO ZF 1 mM; ZnS04 2 mM; TCEP 2 mM

sample_conditions_1: ionic strength: 0 M; pH: 7.3; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D purged-COSY	sample_1	isotropic	sample_conditions_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v2.2, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView v5.03, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

ARIA v1.2, Linge, O'Donoghue and Nilges - refinement, structure solution

NMR spectrometers:

Varian INOVA 600 MHz

PDB	2JVX
DBJ	BAC29672 BAE32372 BAG10959
EMBL	CAB93146
GB	AAC36330 AAC40153 AAD12183 AAD38081 AAF99679
REF	NP_001093326 NP_001093327 NP_001129539 NP_001138727 NP_001154893
SP	O88522 Q6TMG5 Q9Y6K9
AlphaFold	O88522 Q6TMG5 Q9Y6K9