BMRB Entry 15468

Title:
Solution NMR structure of protein NE1242 from N. europaea. Northeast Structural Genomics Target NeT4
Deposition date:
2007-09-12
Original release date:
2007-10-19
Authors:
Wu, Yibing; Yee, Adelinda; Zeri, Ana; Guido, Valerie; Sukumaran, Dinesh; Arrowsmith, Cheryl; Szyperski, Thomas
Citation:

Citation: Wu, Yibing; Yee, Adelinda; Zeri, Ana; Guido, Valerie; Sukumaran, Dinesh; Arrowsmith, Cheryl; Szyperski, Thomas. "Solution NMR structure of protein NE1242 from N. europaea. Northeast Structural Genomics Target NeT4"  .

Assembly members:

Assembly members:
NE1242, polymer, 73 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Neurospora crassa   Taxonomy ID: 5141   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Neurospora crassa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: BL21 DE3

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts311
15N chemical shifts76
1H chemical shifts503

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NE12421

Entities:

Entity 1, NE1242 73 residues - Formula weight is not available

1   METTHRHISHISTHRGLUVALPHEGLUGLY
2   GLYTHRILEASPILEGLUASPASPTHRSER
3   LEUTHRILEASNGLYLYSGLUILESERTYR
4   VALHISASPALAVALLYSASNLYSTRPSER
5   SERARGTYRLEUPROTYRTHRGLNTYRASP
6   SERLEULEUASPLEUALAARGALAILEILE
7   ARGASPTHRVALGLUPHESERGLYVALLYS
8   GLUGLYSER

Samples:

sample_1: NE1242 0.5 mM

sample_conditions_1: ionic strength: 0.32 M; pH: .; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
4,3D, GFT HNNCABCAsample_1isotropicsample_conditions_1
4,3D, GFT CABCACONNHsample_1isotropicsample_conditions_1
4,3D, GFT HCCH COSYsample_1isotropicsample_conditions_1
3D, 15N-13C RESOLVEDSIMULTANIOUS NOESYsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
4,3D, GFT HABCABCONNHsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

AutoStruct, Huang, Tejero, Powers and Montelione - structure solution

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

XEASY, Bartels et al. - peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

AutoAssign, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
EMBL CAD85153
REF WP_011111828

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks