BMRB Entry 15452

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15452

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Title:
Backbone and sidechain 1H, 15N and 13C assignments of the human G-actin binding protein profilin II
Deposition date:
2007-08-31
Original release date:
2008-01-28
Authors:
Ju, Tingting; Peti, Wolfgang
Citation:

Citation: Ju, Tingting; Peti, Wolfgang. "Backbone and sidechain (1)H, (15)N and (13)C assignments of the human G-actin binding protein profilin IIa"  Biomol. NMR Assignments 1, 205-207 (2007).
PubMed: 19636866

Assembly members:

Assembly members:
profilin_II, polymer, 145 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETM30GST

Entity Sequences (FASTA):

Entity Sequences (FASTA):
profilin_II: GAMGASAGWQSYVDNLMCDG CCQEAAIVGYCDAKYVWAAT AGGVFQSITPIEIDMIVGKD REGFFTNGLTLGAKKCSVIR DSLYVDGDCTMDIRTKSQGG EPTYNVAVGRAGRVLVFVMG KEGVHGGGLNKKAYSMAKYL RDSGF

Data sets:
Data typeCount
13C chemical shifts532
15N chemical shifts140
1H chemical shifts828

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1profilin II1

Entities:

Entity 1, profilin II 145 residues - Formula weight is not available

1   GLYALAMETGLYALASERALAGLYTRPGLN
2   SERTYRVALASPASNLEUMETCYSASPGLY
3   CYSCYSGLNGLUALAALAILEVALGLYTYR
4   CYSASPALALYSTYRVALTRPALAALATHR
5   ALAGLYGLYVALPHEGLNSERILETHRPRO
6   ILEGLUILEASPMETILEVALGLYLYSASP
7   ARGGLUGLYPHEPHETHRASNGLYLEUTHR
8   LEUGLYALALYSLYSCYSSERVALILEARG
9   ASPSERLEUTYRVALASPGLYASPCYSTHR
10   METASPILEARGTHRLYSSERGLNGLYGLY
11   GLUPROTHRTYRASNVALALAVALGLYARG
12   ALAGLYARGVALLEUVALPHEVALMETGLY
13   LYSGLUGLYVALHISGLYGLYGLYLEUASN
14   LYSLYSALATYRSERMETALALYSTYRLEU
15   ARGASPSERGLYPHE

Samples:

sample_1: profilin II, [U-99% 13C; U-99% 15N], 1 mM; Na-phosphate 20 mM; NaCl 50 mM

sample_2: profilin II, [U-99% 15N], 0.7 mM; Na-phosphate 20 mM; NaCl 50 mM

sample_3: profilin II 1 mM; Na-phosphate 20 mM; NaCl 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v1.3, Bruker Biospin - collection, processing

XEASY vCARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

PDB
DBJ BAE01927 BAE21281 BAE37648 BAG34725 BAG73581
EMBL CAB87382 CAG31543 CAH91416 CAH92677 CAH93174
GB AAG09756 AAG24949 AAH18049 AAH24363 AAH43646
REF NP_001073228 NP_001121669 NP_001126567 NP_001270511 NP_062283
SP P35080 Q09430 Q4R4P8 Q5R4E2 Q9JJV2
TPG DAA33153
AlphaFold P35080 Q09430 Q4R4P8 Q5R4E2 Q9JJV2

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks