BMRB Entry 15448

Chem Shift validation: LACS
BMRB Entry DOI: doi:10.13018/BMR15448

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for ClpS from Xanthomonas axonopodis pv citri
Deposition date:
2007-08-29
Original release date:
2007-10-01
Authors:
Galvao-Botton, Leonor; Farah, Chuck; Spisni, Alberto; Pertinhez, Thelma
Citation:

Citation: Galvao-Botton, Leonor; Spisni, Alberto; Salinas, Roberto; Farah, Chuck; Pertinhez, Thelma. "NMR structural investigations of the ClpS protein from Xanthomonas axonopodis pv citri"  .

Assembly members:

Assembly members:
ClpS, polymer, 106 residues, 12120.8 Da.

Natural source:

Natural source:   Common Name: Xanthomonas axonopodis pv citri   Taxonomy ID: 53413   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Xanthomonas axonopodis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-3a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ClpS: MPRNTSHEHDHGLMVEASKP EVAPPPRYQVLLLNDDYTPM DFVVTVLEQFFNLNLEQATQ IMLHVHTRGRGVCGVYSREV AESKVAQVNEFSRMNQHPLL CTMEQA

Data sets:
Data typeCount
13C chemical shifts368
15N chemical shifts95
1H chemical shifts537

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ClpS1

Entities:

Entity 1, ClpS 106 residues - 12120.8 Da.

1   METPROARGASNTHRSERHISGLUHISASP
2   HISGLYLEUMETVALGLUALASERLYSPRO
3   GLUVALALAPROPROPROARGTYRGLNVAL
4   LEULEULEUASNASPASPTYRTHRPROMET
5   ASPPHEVALVALTHRVALLEUGLUGLNPHE
6   PHEASNLEUASNLEUGLUGLNALATHRGLN
7   ILEMETLEUHISVALHISTHRARGGLYARG
8   GLYVALCYSGLYVALTYRSERARGGLUVAL
9   ALAGLUSERLYSVALALAGLNVALASNGLU
10   PHESERARGMETASNGLNHISPROLEULEU
11   CYSTHRMETGLUGLNALA

Samples:

sample_1: ClpS, [U-99% 13C; U-99% 15N], 0.5 mM; D2O 5%; sodium phosphate 40 mM; sodium azide 0.025%

sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

NMRView v5.0, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 500 MHz

Related Database Links:

DBJ BAE69168
EMBL CAJ23725 CCF68545 CCG35681 CDF61644 CDN17746
GB AAM36862 AAW75808 ACD59135 ACD59326 AEO42298
REF WP_002813423 WP_005914463 WP_016848737 WP_017158321 WP_019304173
SP Q2P2Q9 Q5GZR3 Q8PL06
AlphaFold Q2P2Q9 Q5GZR3 Q8PL06

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks