BMRB Entry 15445

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15445

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Title:
15N relaxation and H/D exchange analysis of 15.5K free in solution
Deposition date:
2007-08-27
Original release date:
2008-06-26
Authors:
Soss, Sarah; Flynn, Peter
Citation:

Citation: Soss, Sarah; Flynn, Peter. "Functional implications for a prototypical K-turn binding protein from structural and dynamical studies of 15.5K"  Biochemistry 46, 14979-14986 (2007).
PubMed: 18044964

Assembly members:

Assembly members:
15.5K, polymer, 144 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
15.5K: MGHHHHHHSSGIEEGRMTEA DVNPKAYPLADAHLTKKLLD LVQQSCNYKQLRKGANEATK TLNRGISEFIVMAADAEPLE IILHLPLLCEDKNVPYVFVR SKQALGRACGVSRPVIACSV TIKEGSQLKQQIQSIQQSIE RLLV

Data typeCount
heteronuclear NOE values186
H exchange protection factors61
H exchange rates61
order parameters95
T1 relaxation values191
T2 relaxation values189

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
115.5K1

Entities:

Entity 1, 15.5K 144 residues - Formula weight is not available

The first 16 residues are an affinity tag, numbering begins with M1.

1   METGLYHISHISHISHISHISHISSERSER
2   GLYILEGLUGLUGLYARGMETTHRGLUALA
3   ASPVALASNPROLYSALATYRPROLEUALA
4   ASPALAHISLEUTHRLYSLYSLEULEUASP
5   LEUVALGLNGLNSERCYSASNTYRLYSGLN
6   LEUARGLYSGLYALAASNGLUALATHRLYS
7   THRLEUASNARGGLYILESERGLUPHEILE
8   VALMETALAALAASPALAGLUPROLEUGLU
9   ILEILELEUHISLEUPROLEULEUCYSGLU
10   ASPLYSASNVALPROTYRVALPHEVALARG
11   SERLYSGLNALALEUGLYARGALACYSGLY
12   VALSERARGPROVALILEALACYSSERVAL
13   THRILELYSGLUGLYSERGLNLEULYSGLN
14   GLNILEGLNSERILEGLNGLNSERILEGLU
15   ARGLEULEUVAL

Samples:

sample_1: 15.5K, [U-99% 15N], 0.8 mM; D2O 8%; sodium azide 0.1%; sodium chloride 100 mM; sodium phosphate 100 mM

sample_2: 15.5K, [U-99% 15N], 0.6 mM; D2O 95%; H2O 5%; sodium chloride 100 mM; sodium phosphate 100 mM

sample_conditions_1: ionic strength: 200 mM; pH: 6.0; pressure: 1 atm; temperature: 293 K

sample_conditions_2: pD: 6.52; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_2

Software:

FELIX v95.0, Accelrys Software Inc. - peak picking, processing

relxn2.2, Dr. Andrew Lee - processing

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAA23363 BAB23329 BAE01699 BAE22349 BAE26537
EMBL CAG30417 CAG46526 CAK54562 CAK54861
GB AAC72945 AAF06959 AAH05358 AAH19282 AAH26755
PRF 2210268A
REF NP_001003796 NP_001070472 NP_001177155 NP_001177156 NP_001271884
SP P55769 P55770 Q3B8S0 Q4R5C6 Q9D0T1
TPG DAA29093
AlphaFold P55769 P55770 Q3B8S0 Q4R5C6 Q9D0T1