BMRB Entry 15441

Name: Backbone chemical shift assignments of dynein intermediate chain IC74 residues 84-143
Published: 2008-07-03

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15441

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone chemical shift assignments of dynein intermediate chain IC74 residues 84-143

Deposition date:

2007-08-23

Original release date:

2008-07-03

Authors:

Benison, Gregory; Barbar, Elisar; Berkholz, Donald

Citation:

Citation: Benison, Gregory; Berkholz, Donald; Barbar, Elisar. "Protein Assignments without Peak Lists Using Higher-Order Spectra" J. Magn. Reson. 189, 173-181 (2007).
PubMed: 17919953

Assembly members:

Assembly members:
IC74, polymer, 75 residues, Formula weight is not available

Natural source:

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PET15d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
IC74: SHHHHHHSMANSAGSSNTQT PDASLQATVNGQSGGKKQPL NLSVYNVQATNIPPKETLVY TKQTQTTSTGGGNGD

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
heteronucl_NOEs
- heteronuclear_noe_list_1

Data type	Count
13C chemical shifts	111
15N chemical shifts	55
1H chemical shifts	55
heteronuclear NOE values	55

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	IC74	1

Entities:

Entity 1, IC74 75 residues - Formula weight is not available

1

SER

HIS

SER

MET

ALA

2

ASN

SER

ALA

GLY

SER

ASN

THR

GLN

THR

3

PRO

ASP

ALA

SER

LEU

GLN

ALA

THR

VAL

ASN

4

GLY

GLN

SER

GLY

LYS

GLN

PRO

LEU

5

ASN

LEU

SER

VAL

TYR

ASN

VAL

GLN

ALA

THR

6

ASN

ILE

PRO

LYS

GLU

THR

LEU

VAL

TYR

7

THR

LYS

GLN

THR

GLN

THR

SER

THR

GLY

8

GLY

ASN

GLY

ASP

Samples:

sample_1: IC74, [U-100% 13C; U-100% 15N], 1 mM; H2O 90%; D2O 10%; potassium phosphate 50 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 0.25 M; pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-15N hetnoe	sample_1	isotropic	sample_conditions_1

Software:

xwinnmr, Bruker Biospin - collection

Burrow-Owl, Benison, Berkholz, and Barbar - chemical shift assignment, data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Bruker DRX 600 MHz

GB	AAC70933 AAC70934 AAC70935 AAC70938 AAC70939
REF	NP_001285486 NP_001285487 NP_477070 NP_477072 NP_477074
SP	Q24246
AlphaFold	Q24246