BMRB Entry 15439

Name: URN1 FF domain yeast
Published: 2008-06-26

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PDB ID: 2juc
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15439
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

URN1 FF domain yeast

Deposition date:

2007-08-21

Original release date:

2008-06-26

Authors:

Bonet, Roman; Ramirez-Espain, Ximena; Macias, Maria

Citation:

Citation: Bonet, Roman; Ramirez-Espain, Ximena; Macias, Maria. "Solution structure of the yeast URN1 splicing factor FF domain: Comparative analysis of charge distributions in FF domain structures-FFs and SURPs, two domains with a similar fold" Proteins 73, 1001-1009 (2008).
PubMed: 18536009

Assembly members:

Assembly members:
FF, polymer, 58 residues, 6868.629 Da.

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: petm30

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FF: AMGDIDERNIFFELFDRYKL DKFSTWSLQSKKIENDPDFY KIRDDTVRESLFEEWCGE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	397

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity	1

Entities:

Entity 1, entity 58 residues - 6868.629 Da.

1

ALA

MET

GLY

ASP

ILE

ASP

GLU

ARG

ASN

ILE

2

PHE

GLU

LEU

PHE

ASP

ARG

TYR

LYS

LEU

3

ASP

LYS

PHE

SER

THR

TRP

SER

LEU

GLN

SER

4

LYS

ILE

GLU

ASN

ASP

PRO

ASP

PHE

TYR

5

LYS

ILE

ARG

ASP

THR

VAL

ARG

GLU

SER

6

LEU

PHE

GLU

TRP

CYS

GLY

GLU

Samples:

sample_1: FF 0.5 mM; sodium phosphate 20 mM; sodium chloride 130 mM; sodium azide 0.03%; D2O 10%; H2O 90%

sample2: FF, [U-100% 15N], 0.5 mM; sodium phosphate 20 mM; sodium chloride 130 mM; sodium azide 0.03%; D2O 10%; H2O 90%

sample3: FF, [U-100% 13C; U-100% 15N], 0.5 mM; sodium phosphate 20 mM; sodium chloride 130 mM; sodium azide 0.03%; D2O 10%; H2O 90%

sample_conditions_1: pH: 5.8; pressure: 1 atm; temperature: 285 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample2	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample2	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample3	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

CARA, Keller and Wuthrich - data analysis

NMR spectrometers:

Bruker DRX 600 MHz
Bruker DRX 800 MHz

PDB	2JUC
DBJ	GAA27107
EMBL	CAY87107
GB	AAB68289 AAT92759 AFO64407 AFO64408 AFO64409
REF	NP_015478
SP	Q06525
TPG	DAA11564
AlphaFold	Q06525