BMRB Entry 15438

Name: Solution structure of IPI*
Published: 2008-06-26

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PDB ID: 2jub
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15438
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of IPI*

Deposition date:

2007-08-17

Original release date:

2008-06-26

Authors:

Rifat, Dalin; Wright, Nathan; Varney, Kristen; Weber, David; Black, Lindsay

Citation:

Citation: Rifat, Dalin; Wright, Nathan; Varney, Kristen; Weber, David; Black, Lindsay. "Restriction endonuclease inhibitor IPI* of bacteriophage T4: a novel structure for a dedicated target" J. Mol. Biol. 375, 720-734 (2008).
PubMed: 18037438

Assembly members:

Assembly members:
IP1*, polymer, 76 residues, 8060.466 Da.

Natural source:

Natural source: Common Name: T4 phage Taxonomy ID: 10665 Superkingdom: Viruses Kingdom: not available Genus/species: T4-like viruses Enterobacteria phage T4 sensu lato

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
IP1*: ATLTSEVIKANKGREGKPMI SLVDGEEIKGTVYLGDGWSA KKDGATIVISPAEETALFKA KHISAAHLKIIAKNLL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	221
15N chemical shifts	72
1H chemical shifts	452

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	IP1*	1

Entities:

Entity 1, IP1* 76 residues - 8060.466 Da.

1

ALA

THR

LEU

THR

SER

GLU

VAL

ILE

LYS

ALA

2

ASN

LYS

GLY

ARG

GLU

GLY

LYS

PRO

MET

ILE

3

SER

LEU

VAL

ASP

GLY

GLU

ILE

LYS

GLY

4

THR

VAL

TYR

LEU

GLY

ASP

GLY

TRP

SER

ALA

5

LYS

ASP

GLY

ALA

THR

ILE

VAL

ILE

SER

6

PRO

ALA

GLU

THR

ALA

LEU

PHE

LYS

ALA

7

LYS

HIS

ILE

SER

ALA

HIS

LEU

LYS

ILE

8

ILE

ALA

LYS

ASN

LEU

Samples:

sample_1: entity, [U-100% 13C; U-100% 15N], 500 mM; sodium acetate 20 mM; D2O 10%; H2O 90%

sample_conditions_1: ionic strength: 20 mM; pH: 4.8; pressure: 1 atm; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
4D 15N-13C NOESY	sample_1	isotropic	sample_conditions_1
4D 13C-13C NOESY	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Bruker DMX 600 MHz
Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks