BMRB Entry 15435

Name: alpha-RgIA, a Novel Conotoxin That Blocks the alpha9alpha10 nAChR
Published: 2008-03-13

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15435

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

alpha-RgIA, a Novel Conotoxin That Blocks the alpha9alpha10 nAChR

Deposition date:

2007-08-15

Original release date:

2008-03-13

Authors:

Feng, Zhi-Ping

Citation:

Citation: Ellison, Michael; Feng, Zhi-Ping; Park, Anthony; Zhang, Xuecheng; Olivera, Baldomero; McIntosh, J. Michael; Norton, Raymond. "alpha-RgIA, a Novel Conotoxin That Blocks the alpha9alpha10 nAChR: Structure and Identification of Key Receptor-Binding Residues." J. Mol. Biol. 377, 1216-1227 (2008).
PubMed: 18295795

Assembly members:

Assembly members:
RgIA, polymer, 13 residues, 1579.867 Da.

Natural source:

Natural source: Common Name: Conus regius Taxonomy ID: 101314 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Conus regius

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RgIA: GCCSDPRCRYRCR

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	33
15N chemical shifts	11
1H chemical shifts	78

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RgIA	1

Entities:

Entity 1, RgIA 13 residues - 1579.867 Da.

1

GLY

CYS

SER

ASP

PRO

ARG

CYS

ARG

TYR

2

ARG

CYS

ARG

Samples:

sample_1: toxin 1.0 ± 0.2 mM; H2O 95%; D2O 5%

sample_conditions_2: pH: 5.0; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_2
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_2
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_2
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_2
2D DQF-COSY	sample_1	isotropic	sample_conditions_2
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_2

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

NMR spectrometers:

Bruker DRX 600 MHz
Bruker Avance 500 MHz
Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks