BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15435

Title: alpha-RgIA, a Novel Conotoxin That Blocks the alpha9alpha10 nAChR   PubMed: 18295795

Deposition date: 2007-08-15 Original release date: 2008-03-13

Authors: Feng, Zhi-Ping

Citation: Ellison, Michael; Feng, Zhi-Ping; Park, Anthony; Zhang, Xuecheng; Olivera, Baldomero; McIntosh, J. Michael; Norton, Raymond. "alpha-RgIA, a Novel Conotoxin That Blocks the alpha9alpha10 nAChR: Structure and Identification of Key Receptor-Binding Residues."  J. Mol. Biol. 377, 1216-1227 (2008).

Assembly members:
RgIA, polymer, 13 residues, 1579.867 Da.

Natural source:   Common Name: not available   Taxonomy ID: 101314   Superkingdom: Metazoa   Kingdom: Conus   Genus/species: regius not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
RgIA: GCCSDPRCRYRCR

Data sets:
Data typeCount
13C chemical shifts33
15N chemical shifts11
1H chemical shifts78

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1RgIA1

Entities:

Entity 1, RgIA 13 residues - 1579.867 Da.

1   GLYCYSCYSSERASPPROARGCYSARGTYR
2   ARGCYSARG

Samples:

sample_1: toxin 1.0 ± 0.2 mM; H2O 95%; D2O 5%

sample_conditions_2: pH: 5.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_2
2D 1H-15N HSQCsample_1isotropicsample_conditions_2
2D 1H-13C HSQCsample_1isotropicsample_conditions_2
2D 1H-1H TOCSYsample_1isotropicsample_conditions_2
2D DQF-COSYsample_1isotropicsample_conditions_2
2D 1H-1H NOESYsample_1isotropicsample_conditions_2

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker Avance 500 MHz
  • Bruker Avance 800 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts