BMRB Entry 15431

Name: NMR assignment of the periplasmic oxidoreductase DsbH from Chlamydia
Published: 2007-11-16

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PDB ID: 2ju5
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15431
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR assignment of the periplasmic oxidoreductase DsbH from Chlamydia

Deposition date:

2007-08-15

Original release date:

2007-11-16

Authors:

Ulmer, Tobias

Citation:

Citation: Hung, Kuo-Chan; Mac, Thien-Thi; Ulmer, Tobias. "NMR assignment of the periplasmic oxidoreductase DsbH from Chlamydia" Biomol. NMR Assignments 1, 195-196 (2007).
PubMed: 19636863

Assembly members:

Assembly members:
DsbH, polymer, 144 residues, 16487.906 Da.

Natural source:

Natural source: Common Name: Chlamydia pneumoniae Taxonomy ID: 83558 Superkingdom: Bacteria Kingdom: not available Genus/species: Chlamydia pneumoniae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET44-DsbH

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DsbH: ARRRASGENLQQTRPIAAAN LQWESYAEALEHSKQDHKPI GLFFTGSDWCMWCIKMQDQI LQSSEFKHFAGVHLHMVEVD FPQKNHQPEEQRQKNQELKA QYKVTGFPELVFIDAEGKQL ARMGFEPGGGAAYVSKVKSA LKLR

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	582
15N chemical shifts	141
1H chemical shifts	880

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	polypeptide chain	1

Entities:

Entity 1, polypeptide chain 144 residues - 16487.906 Da.

1

ALA

ARG

ALA

SER

GLY

GLU

ASN

LEU

2

GLN

THR

ARG

PRO

ILE

ALA

ASN

3

LEU

GLN

TRP

GLU

SER

TYR

ALA

GLU

ALA

LEU

4

GLU

HIS

SER

LYS

GLN

ASP

HIS

LYS

PRO

ILE

5

GLY

LEU

PHE

THR

GLY

SER

ASP

TRP

CYS

6

MET

TRP

CYS

ILE

LYS

MET

GLN

ASP

GLN

ILE

7

LEU

GLN

SER

GLU

PHE

LYS

HIS

PHE

ALA

8

GLY

VAL

HIS

LEU

HIS

MET

VAL

GLU

VAL

ASP

9

PHE

PRO

GLN

LYS

ASN

HIS

GLN

PRO

GLU

10

GLN

ARG

GLN

LYS

ASN

GLN

GLU

LEU

LYS

ALA

11

GLN

TYR

LYS

VAL

THR

GLY

PHE

PRO

GLU

LEU

12

VAL

PHE

ILE

ASP

ALA

GLU

GLY

LYS

GLN

LEU

13

ALA

ARG

MET

GLY

PHE

GLU

PRO

GLY

14

ALA

TYR

VAL

SER

LYS

VAL

LYS

SER

ALA

15

LEU

LYS

LEU

ARG

Samples:

sample_1: DsbH, [U-99% 13C; U-99% 15N], 1.0 mM; NaH2PO4/Na2HPO4 50 mM; NaN3 0.02 % w/v; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.1 M; pH: 6.0; pressure: 1 atm; temperature: 298.2 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	anisotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

Bruker Avance 700 MHz

PDB	2JU5
DBJ	BAA99134
EMBL	CRI33456 CRI36320 CRI37446 CRI38577 CRI39708
GB	AAD19064 AAF38723 AAP98887 ACZ32813 ETR79697
REF	NP_225121 WP_010883561
SP	Q9Z6Y0
AlphaFold	Q9Z6Y0