BMRB Entry 15422

Name: Partial chemical shift assignments of KChIP4a in its Ca2+- and Mg2+-loaded form
Published: 2007-10-03

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15422

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Partial chemical shift assignments of KChIP4a in its Ca2+- and Mg2+-loaded form

Deposition date:

2007-08-08

Original release date:

2007-10-03

Authors:

Schwenk, Jochen; Kandias, Nikolaos; Neubauer, Isabel; Fakler, Bernd; Bentrop, Detlef

Citation:

Citation: Schwenk, Jochen; Zolles, Gerd; Kandias, Nikolaos; Neubauer, Isabel; Kalbacher, Hubert; Covarrubias, Manuel; Fakler, Bernd; Bentrop, Detlef. "NMR Analysis of KChIP4a Reveals Structural Basis for Control of Surface Expression of Kv4 Channel Complexes" J. Biol. Chem. 283, 18937-18946 (2008).
PubMed: 18458082

Assembly members:

Assembly members:
KChIP4a, polymer, 237 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.
MG, non-polymer, 24.305 Da.

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET16b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
KChIP4a: MGHHHHHHMNLEGLEMIAVL IVIVLFVKLLEQFGLIEAGL EDSVEDELEMATVRHRPEAL ELLEAQSKFTKKELQILYRG FKNECPSGVVNEETFKEIYS QFFPQGDSTTYAHFLFNAFD TDHNGAVSFEDFIKGLSILL RGTVQEKLNWAFNLYDINKD GYITKEEMLDIMKAIYDMMG KCTYPVLKEDAPRQHVETFF QKMDKNKDGVVTIDEFIESC QKDENIMRSMQLFENVI

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	477
15N chemical shifts	161
1H chemical shifts	161

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	KChIP4a	1
2	ligand1	2
3	ligand2	2
4	ligand3	3

Entities:

Entity 1, KChIP4a 237 residues - Formula weight is not available

The first eight residues represent a non-native affinity tag.

1

MET

GLY

HIS

MET

ASN

2

LEU

GLU

GLY

LEU

GLU

MET

ILE

ALA

VAL

LEU

3

ILE

VAL

ILE

VAL

LEU

PHE

VAL

LYS

LEU

4

GLU

GLN

PHE

GLY

LEU

ILE

GLU

ALA

GLY

LEU

5

GLU

ASP

SER

VAL

GLU

ASP

GLU

LEU

GLU

MET

6

ALA

THR

VAL

ARG

HIS

ARG

PRO

GLU

ALA

LEU

7

GLU

LEU

GLU

ALA

GLN

SER

LYS

PHE

THR

8

LYS

GLU

LEU

GLN

ILE

LEU

TYR

ARG

GLY

9

PHE

LYS

ASN

GLU

CYS

PRO

SER

GLY

VAL

10

ASN

GLU

THR

PHE

LYS

GLU

ILE

TYR

SER

11

GLN

PHE

PRO

GLN

GLY

ASP

SER

THR

12

TYR

ALA

HIS

PHE

LEU

PHE

ASN

ALA

PHE

ASP

13

THR

ASP

HIS

ASN

GLY

ALA

VAL

SER

PHE

GLU

14

ASP

PHE

ILE

LYS

GLY

LEU

SER

ILE

LEU

15

ARG

GLY

THR

VAL

GLN

GLU

LYS

LEU

ASN

TRP

16

ALA

PHE

ASN

LEU

TYR

ASP

ILE

ASN

LYS

ASP

17

GLY

TYR

ILE

THR

LYS

GLU

MET

LEU

ASP

18

ILE

MET

LYS

ALA

ILE

TYR

ASP

MET

GLY

19

LYS

CYS

THR

TYR

PRO

VAL

LEU

LYS

GLU

ASP

20

ALA

PRO

ARG

GLN

HIS

VAL

GLU

THR

PHE

21

GLN

LYS

MET

ASP

LYS

ASN

LYS

ASP

GLY

VAL

22

VAL

THR

ILE

ASP

GLU

PHE

ILE

GLU

SER

CYS

23

GLN

LYS

ASP

GLU

ASN

ILE

MET

ARG

SER

MET

24

GLN

LEU

PHE

GLU

ASN

VAL

ILE

Entity 2, ligand1 - Ca - 40.078 Da.

1

CA

Entity 3, ligand3 - Mg - 24.305 Da.

1

MG

Samples:

sample_1: KChIP4a, [U-13C; U-15N; U-2H], 0.1 – 0.25 mM; MOPS 10 mM; DTT 4 mM; N-octylglucoside 5 mM; magnesium chloride 2 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v1.3, Bruker Biospin - collection, processing

CARA v1.5, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz

PDB	3DD4
DBJ	BAC30218 BAE21645 BAE28641 BAE38374 BAE90434
EMBL	CAH90097
GB	AAG36974 AAG36976 AAG36977 AAH32520 AAH51130
REF	NP_001030175 NP_001030176 NP_001070403 NP_001127236 NP_001186171
SP	Q2KI69 Q6PHZ8 Q6PIL6 Q8HYN7 Q99MG9
TPG	DAA28811
AlphaFold	Q2KI69 Q6PHZ8 Q6PIL6 Q8HYN7 Q99MG9