BMRB Entry 15412

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Snu13p
Published: 2007-12-10

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15412

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for Snu13p

Deposition date:

2007-07-31

Original release date:

2007-12-10

Authors:

Workman, Hillary; Skalicky, Jack; Flynn, Peter

Citation:

Citation: Workman, Hillary; Skalicky, Jack; Flynn, Peter. "Assignment of 1H, 13C, and 15N resonances of the RNA binding protein Snu13p from Saccharomyces cerevisiae" Biomol. NMR Assignments 2, 1-3 (2008).
PubMed: 19636910

Assembly members:

Assembly members:
Snu13p, polymer, 136 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Snu13p: MASSAPNPKAFPLADAALTQ QILDVVQQAANLRQLKKGAN EATKTLNRGISEFIIMAADC EPIEILLHLPLLCEDKNVPY VFVPSRVALGRACGVSRPVI AASITTNDASAIKTQIYAVK DKIETLLILEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	549
15N chemical shifts	130
1H chemical shifts	900

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Snu13p	1

Entities:

Entity 1, Snu13p 136 residues - Formula weight is not available

1

MET

ALA

SER

ALA

PRO

ASN

PRO

LYS

ALA

2

PHE

PRO

LEU

ALA

ASP

ALA

LEU

THR

GLN

3

GLN

ILE

LEU

ASP

VAL

GLN

ALA

4

ASN

LEU

ARG

GLN

LEU

LYS

GLY

ALA

ASN

5

GLU

ALA

THR

LYS

THR

LEU

ASN

ARG

GLY

ILE

6

SER

GLU

PHE

ILE

MET

ALA

ASP

CYS

7

GLU

PRO

ILE

GLU

ILE

LEU

HIS

LEU

PRO

8

LEU

CYS

GLU

ASP

LYS

ASN

VAL

PRO

TYR

9

VAL

PHE

VAL

PRO

SER

ARG

VAL

ALA

LEU

GLY

10

ARG

ALA

CYS

GLY

VAL

SER

ARG

PRO

VAL

ILE

11

ALA

SER

ILE

THR

ASN

ASP

ALA

SER

12

ALA

ILE

LYS

THR

GLN

ILE

TYR

ALA

VAL

LYS

13

ASP

LYS

ILE

GLU

THR

LEU

ILE

LEU

GLU

14

HIS

Samples:

sample_1: Snu13p, [U-100% 13C; U-100% 15N], 1 mM; sodium acetate 50 mM; sodium chloride 50 mM; D2O 10%

sample_conditions_1: ionic strength: 100 mM; pH: 5.5; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HCCH-COSY	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D 13C-15N HSQC-NOESY	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Varian INOVA 600 MHz

PDB	1ZWZ 2ALE 4NUT
DBJ	GAA22798
EMBL	CAY79137 CCC67393 CCF58728 CCK73464
GB	AAB64503 AAS56703 AHY75526 AJP38240 AJU39930
REF	NP_010888 XP_003673774 XP_003957863 XP_003980140
SP	P39990
TPG	DAA07626
AlphaFold	P39990