BMRB Entry 15409

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for the cytoplasmic domain of the T cell receptor zeta chain
Published: 2008-03-14

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15409

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for the cytoplasmic domain of the T cell receptor zeta chain

Deposition date:

2007-07-30

Original release date:

2008-03-14

Authors:

Jaravine, Victor; Zhuravleva, Anastasia; Permi, Perttu; Ibraghimov, Ilgis; Orekhov, Vladislav

Citation:

Citation: Jaravine, Victor; Zhuravleva, Anastasia; Permi, Perttu; Ibraghimov, Ilgis; Orekhov, Vladislav. "Hyperdimensional NMR Spectroscopy with Nonlinear Sampling." J. Am. Chem. Soc. 130, 3927-3936 (2008).
PubMed: 18311971

Assembly members:

Assembly members:
T-cell_receptor_zeta-chain, polymer, 115 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET32a(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
T-cell_receptor_zeta-chain: GSLRVKFSRSADAPAYQQGQ NQLYNELNLGRREEYDVLDK RRGRDPEMGGKPRRKNPQEG LYNELQKDKMAEAYSEIGMK GERRRGKGHDGLYQGLSTAT KDTYDALHMQALPPR

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	321
15N chemical shifts	104
1H chemical shifts	104

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	subunit 1	1

Entities:

Entity 1, subunit 1 115 residues - Formula weight is not available

1

GLY

SER

LEU

ARG

VAL

LYS

PHE

SER

ARG

SER

2

ALA

ASP

ALA

PRO

ALA

TYR

GLN

GLY

GLN

3

ASN

GLN

LEU

TYR

ASN

GLU

LEU

ASN

LEU

GLY

4

ARG

GLU

TYR

ASP

VAL

LEU

ASP

LYS

5

ARG

GLY

ARG

ASP

PRO

GLU

MET

GLY

6

LYS

PRO

ARG

LYS

ASN

PRO

GLN

GLU

GLY

7

LEU

TYR

ASN

GLU

LEU

GLN

LYS

ASP

LYS

MET

8

ALA

GLU

ALA

TYR

SER

GLU

ILE

GLY

MET

LYS

9

GLY

GLU

ARG

GLY

LYS

GLY

HIS

ASP

10

GLY

LEU

TYR

GLN

GLY

LEU

SER

THR

ALA

THR

11

LYS

ASP

THR

TYR

ASP

ALA

LEU

HIS

MET

GLN

12

ALA

LEU

PRO

ARG

Samples:

sample_1: T-cell receptor zeta-chain, [U-13C; U-15N], 0.5 mM; H2O 90%; D2O 10%; Phosphate buffer 20 mM

sample_conditions_1: ionic strength: 0.02 M; pH: 6.7; pressure: 1 atm; temperature: 288 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D iHNCA	sample_1	isotropic	sample_conditions_1
3D iHN(CA)CB	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe v2.6, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - peak picking, processing

AutoAssign v2.3.0, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment

MDD v1.3, (MDDnmr) V.Orekhov, V.Jaravine - data analysis, processing

NMR spectrometers:

Varian INOVA 900 MHz

DBJ	BAC87407 BAD97045 BAG36664 BAJ20574
GB	AAA60394 AAD34640 AAF34793 AAH25703 AAY57330
REF	NP_000725 NP_932170 XP_001174731 XP_003258862 XP_003824647
SP	P20963 Q9XSJ9
AlphaFold	P20963 Q9XSJ9