BMRB Entry 15406

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15406

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Title:
Solution structure of E.coli PapI
Deposition date:
2007-07-26
Original release date:
2008-06-26
Authors:
Kawamura, T.; Zhou, H.; Le, L.; Dahlquist, F.
Citation:

Citation: Kawamura, T.; Le, L.; Zhou, H.; Dahlquist, F.. "Solution structure of E.coli PapI, a key regulator of the pap pili phase variation"  J. Mol. Biol. 365, 1130-1142 (2007).
PubMed: 17109885

Assembly members:

Assembly members:
P_fimbrial_regulatory_protein_KS71A, polymer, 81 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
P_fimbrial_regulatory_protein_KS71A: MKNEILEFLNRHNGGKTAEI AEALAVTDYQARYYLLLLEK AGMVQRSPLRRGMATYWFLK GEKQAGQSCSSTTLEHHHHH H

Data sets:
Data typeCount
13C chemical shifts335
15N chemical shifts76
1H chemical shifts519

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PapI monomer1

Entities:

Entity 1, PapI monomer 81 residues - Formula weight is not available

1   METLYSASNGLUILELEUGLUPHELEUASN
2   ARGHISASNGLYGLYLYSTHRALAGLUILE
3   ALAGLUALALEUALAVALTHRASPTYRGLN
4   ALAARGTYRTYRLEULEULEULEUGLULYS
5   ALAGLYMETVALGLNARGSERPROLEUARG
6   ARGGLYMETALATHRTYRTRPPHELEULYS
7   GLYGLULYSGLNALAGLYGLNSERCYSSER
8   SERTHRTHRLEUGLUHISHISHISHISHIS
9   HIS

Samples:

sample_1: P fimbrial regulatory protein KS71A, [U-99% 13C; U-99% 15N], 400 uM; potassium phosphate, none, 50 mM; potassium chloride, none, 150 mM; beta-mercaptoethanol, none, 5 mM; EDTA, none, 100 uM; D2O, [U-99% 2H], 8 %v/v; sodium azide, none, 0.02 %w/v

sample_2: P fimbrial regulatory protein KS71A, [U-99% 13C; U-99% 15N], 400 uM; potassium phosphate, none, 50 mM; potassium chloride, none, 150 mM; DTT, none, 2 mM; D2O, [U-99% 2H], 99%

sample_3: P fimbrial regulatory protein KS71A, [U-99% 15N], 400 uM; potassium phosphate, none, 50 mM; potassium chloride, none, 150 mM; EDTA, none, 100 uM; beta-mercaptoethanol, none, 5 mM; D2O, none, 8 %v/v; sodium azide, none, 0.02 %w/v

sample_conditions_1: ionic strength: 150 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
4D_13C-separated_NOESYsample_2isotropicsample_conditions_1
4D_13C/15N-separated_NOESYsample_1isotropicsample_conditions_1
HNHAsample_3isotropicsample_conditions_1
3D_15N-separated_NOESYsample_3isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D CCC-TOCSYsample_1isotropicsample_conditions_1

Software:

X-PLOR v3.851, Brunger - refinement

ansig4opengl v1.0.4, Per Kraulis - data analysis

NMRPipe, Frank Delaglio - processing

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
EMBL CAA32630 CAA32633 CAR04501 CAR14504 CAR20684
GB AAA24048 AAA92572 AAC95391 AAL67419 AAN82041
REF WP_000006206 WP_000006207 WP_000006208 WP_020232424 WP_032199237
SP P62584 P62585
AlphaFold P62584 P62585

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks