BMRB Entry 15404

Title:
Backbone and sidechain 1H, 13C, and 15N Chemical Shift Assignments for H. capsulatum CBP
Deposition date:
2007-07-23
Original release date:
2007-08-13
Authors:
Beck, Moriah; DeKoster, Gregory; Cistola, David; Goldman, William
Citation:

Citation: Beck, Moriah; DeKoster, Gregory; Hambly, David; Gross, Micheal; Cistola, David; Goldman, William. "Structural features responsible for the biological stability of Histoplasma's virulence factor CBP"  Biochemistry 47, 4427-4438 (2008).
PubMed: 18361504

Assembly members:

Assembly members:
CBP, polymer, 78 residues, 7855 Da.

Natural source:

Natural source:   Common Name: Ajellomyces capsulata   Taxonomy ID: 5037   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Histoplasma capsulatum

Experimental source:

Experimental source:   Production method: purified from the natural source   Host organism: Histoplasma capsulatum   Vector: pTS404

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts276
15N chemical shifts80
1H chemical shifts455
coupling constants66

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CBP, monomer 11
2CBP, monomer 21

Entities:

Entity 1, CBP, monomer 1 78 residues - 7855 Da.

G186AR strain mature peptide

1   ASPGLNPROSERVALGLYASPALAPHEASP
2   LYSTYRASNGLUALAVALARGVALPHETHR
3   GLNLEUSERSERALAALAASNCYSASPTRP
4   ALAALACYSLEUSERSERLEUSERALASER
5   SERALAALACYSILEALAALAVALGLYGLU
6   LEUGLYLEUASPVALPROLEUASPLEUALA
7   CYSALAALATHRALATHRSERSERALATHR
8   GLUALACYSLYSGLYCYSLEUTRP

Samples:

CBP-13C_15N: CBP, [U-99% 13C; U-99% 15N], 2 ± 1 mM; D2O, [U-99% 2H], 5%; H2O 95%; potassium chloride 100 mM; HEPES, [U-98% 2H], 10 mM; sodium azide 0.05%

CBP-15N: CBP, [U-99% 15N], 1 ± 0.2 mM; D2O, [U-99% 2H], 5%; H2O 95%; potassium chloride 100 mM; HEPES, [U-98% 2H], 10 mM; sodium azide 0.05%

CBP-13C_15N-cys: CBP, [U-99% 13C; U-99% 15N], 2 ± 1 mM; D2O, [U-99% 2H], 5%; H2O 95%; potassium chloride 100 mM; HEPES, [U-98% 2H], 10 mM; sodium azide 0.05%

CBP-normal: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCCBP-15NisotropicCBP-normal
3D HNCACBCBP-13C_15NisotropicCBP-normal
3D CBCA(CO)NHCBP-13C_15N-cysisotropicCBP-normal
3D HBHA(CO)NHCBP-13C_15N-cysisotropicCBP-normal
3D HNCOCBP-13C_15NisotropicCBP-normal
3D CBCA(CO)CAHACBP-13C_15NisotropicCBP-normal
3D HCCH-TOCSYCBP-13C_15NisotropicCBP-normal
3D HCC(TOCSY)NNHCBP-13C_15NisotropicCBP-normal
3D CC(TOCSY)NNHCBP-13C_15NisotropicCBP-normal
3D aromatic HC(C)H-TOCSYCBP-13C_15NisotropicCBP-normal
2D aromatic 13C-HSQCCBP-13C_15NisotropicCBP-normal
2D hbCBcgcdHDCBP-13C_15NisotropicCBP-normal
2D hbCBcgcdHDHECBP-13C_15NisotropicCBP-normal
3D 1H-13C NOESYCBP-13C_15N-cysisotropicCBP-normal
3D HNHACBP-13C_15N-cysisotropicCBP-normal

Software:

FELIX v2001, Accelrys Software Inc. - chemical shift assignment, peak picking, processing

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 700 MHz
  • Varian INOVA 500 MHz

Related Database Links:

SWS O42720

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks