BMRB Entry 15401
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15401
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Citation: Hu, Yunfei; Zhang, Xinxin; Shi, Yunming; Zhou, Yanfeng; Zhang, Wei; Su, Xiao-Dong; Xia, Bin; Zhao, Jindong; Jin, Changwen. "Structures of Anabaena Calcium-binding Protein CcbP: INSIGHTS INTO CA2+ SIGNALING DURING HETEROCYST DIFFERENTIATION." J. Biol. Chem. 286, 12381-12388 (2011).
PubMed: 21330362
Assembly members:
Alr1010_protein, polymer, 126 residues, Formula weight is not available
Natural source: Common Name: Anabaena sp. Taxonomy ID: 1167 Superkingdom: Bacteria Kingdom: not available Genus/species: Anabaena Anabaena sp.
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b
Entity Sequences (FASTA):
Alr1010_protein: MASVERDETREHRIETEIIV
DAEDKEERAMGWYYYLDDTL
EFPFMGKWKKKSRKTSTIEE
KTVEVLGMAPDDECLKDMYV
EVADIGGKDDDVYTAKLSDI
EAIDVDDDTQEAIADWLYWL
ARGYKF
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 532 |
| 15N chemical shifts | 127 |
| 1H chemical shifts | 805 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ccbp | 1 |
Entities:
Entity 1, ccbp 126 residues - Formula weight is not available
| 1 | MET | ALA | SER | VAL | GLU | ARG | ASP | GLU | THR | ARG | ||||
| 2 | GLU | HIS | ARG | ILE | GLU | THR | GLU | ILE | ILE | VAL | ||||
| 3 | ASP | ALA | GLU | ASP | LYS | GLU | GLU | ARG | ALA | MET | ||||
| 4 | GLY | TRP | TYR | TYR | TYR | LEU | ASP | ASP | THR | LEU | ||||
| 5 | GLU | PHE | PRO | PHE | MET | GLY | LYS | TRP | LYS | LYS | ||||
| 6 | LYS | SER | ARG | LYS | THR | SER | THR | ILE | GLU | GLU | ||||
| 7 | LYS | THR | VAL | GLU | VAL | LEU | GLY | MET | ALA | PRO | ||||
| 8 | ASP | ASP | GLU | CYS | LEU | LYS | ASP | MET | TYR | VAL | ||||
| 9 | GLU | VAL | ALA | ASP | ILE | GLY | GLY | LYS | ASP | ASP | ||||
| 10 | ASP | VAL | TYR | THR | ALA | LYS | LEU | SER | ASP | ILE | ||||
| 11 | GLU | ALA | ILE | ASP | VAL | ASP | ASP | ASP | THR | GLN | ||||
| 12 | GLU | ALA | ILE | ALA | ASP | TRP | LEU | TYR | TRP | LEU | ||||
| 13 | ALA | ARG | GLY | TYR | LYS | PHE |
Samples:
sample: Alr1010 protein, [U-15N; U-13C], 1 mM; Tris 20 mM; NaCl 220 mM; DTT 10 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 220 mM; pH: 7.5; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D_13C-separated_NOESY | sample | isotropic | sample_conditions_1 |
| 3D_15N-separated_NOESY | sample | isotropic | sample_conditions_1 |
| HNCACB,CBCA(CO)NH | sample | isotropic | sample_conditions_1 |
| HNCO,HN(CA)CO | sample | isotropic | sample_conditions_1 |
| HC(C)H-COSY | sample | isotropic | sample_conditions_1 |
| HC(C)H-TOCSY | sample | isotropic | sample_conditions_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v2.1, F.Delaglio, S.Grzesiek, G.W.Vuister, G.Zhu, J.Pfeifer, A.Bax - processing
NMRView v5, B.A.Johnson, R.A.Blevins - data analysis
DYANA vcyana2.0, P.Guntert, C.Mumenthaler, Wuthrich, K.W.Thrich - structure solution
AMBER v7.0, D.A.Case - refinement
NMR spectrometers:
- Bruker AVANCE 800 MHz
- Bruker AVANCE 500 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks