BMRB Entry 15383

Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15383

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Title:
1H, 13C, and 15N Chemical Shift Assignments for the N-domain of Bacillus subtilis ClpC
Deposition date:
2007-07-17
Original release date:
2007-09-17
Authors:
Kojetin, Douglas; McLaughlin, Patrick; Thompson, Richele; Venters, Ronald; Rance, Mark; Cavanagh, John
Citation:

Citation: Kojetin, Douglas; McLaughlin, Patrick; Thompson, Richele; Venters, Ronald; Rance, Mark; Cavanagh, John. "NMR assignment of the N-terminal repeat domain of Bacillus subtilis ClpC"  Biomol. NMR Assignments 1, 163-165 (2007).
PubMed: 19636855

Assembly members:

Assembly members:
NClpCR, polymer, 146 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-16b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NClpCR: HMMFGRFTERAQKVLALAQE EALRLGHNNIGTEHILLGLV REGEGIAAKALQALGLGSEK IQKEVESLIGRGQEMSQTIH YTPRAKKVIELSMDEARKLG HSYVGTEHILLGLIREGEGV AARVLNNLGVSLNKARQQVL QLLGSN

Data sets:
Data typeCount
13C chemical shifts621
15N chemical shifts169
1H chemical shifts1017

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ClpC N-domain1

Entities:

Entity 1, ClpC N-domain 146 residues - Formula weight is not available

Residue H1 is a cloning artifact from Factor Xa cleavage. NMR assignments start from the first M residue. Hereafter, the residue number should be H-1 and M1.

1   HISMETMETPHEGLYARGPHETHRGLUARG
2   ALAGLNLYSVALLEUALALEUALAGLNGLU
3   GLUALALEUARGLEUGLYHISASNASNILE
4   GLYTHRGLUHISILELEULEUGLYLEUVAL
5   ARGGLUGLYGLUGLYILEALAALALYSALA
6   LEUGLNALALEUGLYLEUGLYSERGLULYS
7   ILEGLNLYSGLUVALGLUSERLEUILEGLY
8   ARGGLYGLNGLUMETSERGLNTHRILEHIS
9   TYRTHRPROARGALALYSLYSVALILEGLU
10   LEUSERMETASPGLUALAARGLYSLEUGLY
11   HISSERTYRVALGLYTHRGLUHISILELEU
12   LEUGLYLEUILEARGGLUGLYGLUGLYVAL
13   ALAALAARGVALLEUASNASNLEUGLYVAL
14   SERLEUASNLYSALAARGGLNGLNVALLEU
15   GLNLEULEUGLYSERASN

Samples:

sample_1: NClpCR, [U-13C; U-15N], 0.75 ± 0.25 mM; MES 20 mM; sodium chloride 50 mM; EDTA 2 mM; D2O 10%; H2O 90%

sample_2: NClpCR, [U-15N], 0.75 ± 0.25 mM; MES 20 mM; sodium chloride 50 mM; EDTA 2 mM; D2O 10%; H2O 90%

sample_3: NClpCR, [U-10% 13C], 0.75 ± 0.25 mM; MES 20 mM; sodium chloride 50 mM; EDTA 2 mM; D2O 10%; H2O 90%

sample_conditions_1: ionic strength: 50 mM; pH: 5.5; pressure: 1 atm; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CA)CBsample_1isotropicsample_conditions_1
3D CB(CACO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HNHAsample_2isotropicsample_conditions_1
3D HNHBsample_2isotropicsample_conditions_1
4D 1H-13C-15N-1H NOESYsample_1isotropicsample_conditions_1
4D 1H-13C-13C-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_3isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - data analysis, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Varian INOVA 600 MHz
  • Varian INOVA 500 MHz

Related Database Links:

BMRB 15910
PDB
GB ALC83305
REF WP_053605144

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks