BMRB Entry 15377

Name: Solution structure of human DESR1
Published: 2008-03-13

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PDB ID: 2jr7
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15377
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of human DESR1

Deposition date:

2007-07-12

Original release date:

2008-03-13

Authors:

Wu, Fangming; Wu, Jihui; Shi, Yunyu

Citation:

Citation: Wu, Fangming; Zhang, J.; Sun, J.; Huang, H.; Ji, P.; Chu, W.; Yu, M.; Yang, F.; Wu, Z.; Wu, J.; Shi, Y.. "Solution structure of human DESR1, a CSL zinc-binding protein." Proteins 71, 514-518 (2008).
PubMed: 18214955

Assembly members:

Assembly members:
DESR1, polymer, 89 residues, 9512.521 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET22b (+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DESR1: MAVFHDEVEIEDFQYDEDSE TYFYPCPCGDNFSITKEDLE NGEDVATCPSCSLIIKVIYD KDQFVSGETVPAPSANKELV KLEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	317
15N chemical shifts	76
1H chemical shifts	535

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	DESR1	1
2	ZN	2

Entities:

Entity 1, DESR1 89 residues - 9512.521 Da.

1

MET

ALA

VAL

PHE

HIS

ASP

GLU

VAL

GLU

ILE

2

GLU

ASP

PHE

GLN

TYR

ASP

GLU

ASP

SER

GLU

3

THR

TYR

PHE

TYR

PRO

CYS

PRO

CYS

GLY

ASP

4

ASN

PHE

SER

ILE

THR

LYS

GLU

ASP

LEU

GLU

5

ASN

GLY

GLU

ASP

VAL

ALA

THR

CYS

PRO

SER

6

CYS

SER

LEU

ILE

LYS

VAL

ILE

TYR

ASP

7

LYS

ASP

GLN

PHE

VAL

SER

GLY

GLU

THR

VAL

8

PRO

ALA

PRO

SER

ALA

ASN

LYS

GLU

LEU

VAL

9

LYS

LEU

GLU

HIS

Entity 2, ZN - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: entity_1, [U-99% 13C; U-99% 15N], 1.5 mM; TRIS 20 mM; sodium chloride 50 mM; beta-mercaptoethanol 14 mM; H2O 90%; D2O 10%

sample_2: entity_1, [U-99% 13C; U-99% 15N], 1.5 mM; TRIS 20 mM; sodium chloride 50 mM; beta-mercaptoethanol 14 mM; D2O 99.9%

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 295 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_2	isotropic	sample_conditions_1

Software:

CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMRPipe v2.2, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY v3, Goddard - data analysis

CSI v1.0, David S. Wishart - data analysis

Molmol v2K.2, Koradi, Billeter and Wuthrich - data analysis

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

Bruker DMX 600 MHz

PDB	2JR7
DBJ	BAE02507
EMBL	CAH92222
GB	AAH10181 AAI68674 ADZ15684 AIC53581 EAW64268
REF	NP_001126299 NP_001247449 NP_996662 XP_002759684 XP_002814073
SP	Q4R312 Q5R7N8 Q96FX2
AlphaFold	Q4R312 Q5R7N8 Q96FX2