BMRB Entry 15366

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR15366

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Title:
Human eRF1 C-domain
Deposition date:
2007-07-05
Original release date:
2008-01-28
Authors:
Mantsyzov, Alexey; Polshakov, Vladimir; Birdsall, Berry; Kisselev, Lev
Citation:

Citation: Mantsyzov, Alexey; Ivanova, Elena; Birdsall, Berry; Kolosov, Petr; Kisselev, Lev; Polshakov, Vladimir. "NMR assignments of the C-terminal domain of human polypeptide release factor eRF1"  Biomol. NMR Assignments 1, 183-185 (2007).
PubMed: 19636860

Assembly members:

Assembly members:
C-domain_of_human_polypeptide_release_factor_eRF1, polymer, 171 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET23b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
C-domain_of_human_polypeptide_release_factor_eRF1: MSNVKFIQEKKLIGRYFDEI SQDTGKYCFGVEDTLKALEM GAVEILIVYENLDIMRYVLH CQGTEEEKILYLTPEQEKDK SHFTDKETGQEHELIESMPL LEWFANNYKKFGATLEIVTD KSQEGSQFVKGFGGIGGILR YRVDFQGMEYQGGDDEFFDL DDYLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts315
15N chemical shifts160
1H chemical shifts160

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Human eRF1 C-domain1

Entities:

Entity 1, Human eRF1 C-domain 171 residues - Formula weight is not available

1   METSERASNVALLYSPHEILEGLNGLULYS
2   LYSLEUILEGLYARGTYRPHEASPGLUILE
3   SERGLNASPTHRGLYLYSTYRCYSPHEGLY
4   VALGLUASPTHRLEULYSALALEUGLUMET
5   GLYALAVALGLUILELEUILEVALTYRGLU
6   ASNLEUASPILEMETARGTYRVALLEUHIS
7   CYSGLNGLYTHRGLUGLUGLULYSILELEU
8   TYRLEUTHRPROGLUGLNGLULYSASPLYS
9   SERHISPHETHRASPLYSGLUTHRGLYGLN
10   GLUHISGLULEUILEGLUSERMETPROLEU
11   LEUGLUTRPPHEALAASNASNTYRLYSLYS
12   PHEGLYALATHRLEUGLUILEVALTHRASP
13   LYSSERGLNGLUGLYSERGLNPHEVALLYS
14   GLYPHEGLYGLYILEGLYGLYILELEUARG
15   TYRARGVALASPPHEGLNGLYMETGLUTYR
16   GLNGLYGLYASPASPGLUPHEPHEASPLEU
17   ASPASPTYRLEUGLUHISHISHISHISHIS
18   HIS

Samples:

sample_1: C-domain of human polypeptide release factor eRF1 1 mM; potassium phosphate 10 mM; potassium chloride 50 mM

sample_2: C-domain of human polypeptide release factor eRF1, [U-99% 15N], 1.1 mM; potassium phosphate 10 mM; potassium chloride 20 mM

sample_3: C-domain of human polypeptide release factor eRF1, [U-99% 13C; U-99% 15N], 0.8 mM; potassium phosphate 10 mM; potassium chloride 20 mM

sample_conditions_1: ionic strength: 0.08 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_3isotropicsample_conditions_1
3D HNCAsample_3isotropicsample_conditions_1
3D HNCACBsample_3isotropicsample_conditions_1
3D HN(CO)CAsample_3isotropicsample_conditions_1
3D CBCA(CO)NHsample_3isotropicsample_conditions_1
3D HBHA(CO)NHsample_3isotropicsample_conditions_1
3D HNCACBsample_3isotropicsample_conditions_1
3D HNCOsample_3isotropicsample_conditions_1
3D HNHAsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
3D 1H-13C NOESYsample_3isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

VNMR, Varian - collection

xwinnmr, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - data analysis

AutoAssign, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment

Pence, Wishart and Sykes - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

BMRB 19506
PDB
DBJ BAA13439 BAA85489 BAC33839 BAE31210 BAE31619
EMBL CAA57281 CAA57282 CAH93389
GB AAB49726 AAD43966 AAH13717 AAH14269 AAH85902
REF NP_001008345 NP_001069722 NP_001076236 NP_001126989 NP_001239075
SP P62495 P62496 P62497 Q0VCX5 Q5R4C7
TPG DAA27419
AlphaFold P62495 P62496 P62497 Q0VCX5 Q5R4C7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks