BMRB Entry 15363

Title:
A D-amino acid containing conopeptide, marmophine, from Conus marmoreus
Deposition date:
2007-07-04
Original release date:
2008-06-25
Authors:
Huang, F.; Du, W.; Han, Y.; Wang, C.
Citation:

Citation: Han, Y.; Huang, F.; Jiang, H.; Liu, L.; Wang, Q.; Wang, Y.; Shao, X.; Chi, C.; Du, W.; Wang, C.. "Purification and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus"  FEBS. J. 275, 1976-1987 (2008).
PubMed: 18355315

Assembly members:

Assembly members:
M-conotoxin_mr12, polymer, 15 residues, 1935.080 Da.

Natural source:

Natural source:   Common Name: Conus marmoreus   Taxonomy ID: 42752   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Conus marmoreus

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
M-conotoxin_mr12: DWEYHAHPKXNSXWT

Data sets:
Data typeCount
1H chemical shifts82

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1chain A1

Entities:

Entity 1, chain A 15 residues - 1935.080 Da.

1   ASPTRPGLUTYRHISALAHISPROLYSHYP
2   ASNSERDPNTRPTHR

Samples:

sample_1: M-conotoxin mr12 2.0 mM; TFA 0.01%; H2O 90%; D2O 10%

sample_2: M-conotoxin mr12 2.0 mM; TFA 0.01%; D2O 99.99%

sample_conditions_1: pH: 3; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1isotropicsample_conditions_1
2D TOCSYsample_1isotropicsample_conditions_1
DQF-COSYsample_1isotropicsample_conditions_1
2D NOESYsample_2isotropicsample_conditions_1
2D TOCSYsample_2isotropicsample_conditions_1
DQF-COSYsample_2isotropicsample_conditions_1

Software:

xwinnmr v3.5, Bruker Inc. - collection

TOPSPIN v1.3B, Bruker Inc. - processing

SPARKY v3.110, T. D. Goddard and D. G. Kneller - structure solution

CYANA v2.1, L. A. Systems, Inc. - structure solution

AMBER v9.0, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Koll - refinement

NMR spectrometers:

  • Bruker AVANCE 600 MHz

Related Database Links:

PDB