BMRB Entry 15359
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15359
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Andra, J.; Jakovkin, Igor; Groetzinger, J.; Hecht, Oliver; Krasnosdembskaya, Anna; Goldman, T.; Gutsmann, T.; Leippe, Matthias. "Structure and mode of action of the antimicrobial peptide arenicin" Biochem. J. 410, 113-122 (2008).
PubMed: 17935487
Assembly members:
arenicin monomer, polymer, 21 residues, 2766.365 Da.
Natural source: Common Name: lugworm Taxonomy ID: 6344 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Arenicola marina
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
arenicin monomer: RWCVYAYVRVRGVLVRYRRC
W
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 143 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | arenicin monomer | 1 |
Entities:
Entity 1, arenicin monomer 21 residues - 2766.365 Da.
| 1 | ARG | TRP | CYS | VAL | TYR | ALA | TYR | VAL | ARG | VAL | ||||
| 2 | ARG | GLY | VAL | LEU | VAL | ARG | TYR | ARG | ARG | CYS | ||||
| 3 | TRP |
Samples:
sample_1: entity 1.8 mg; acetic acid, [U-2H], 2%
sample_conditions_1: pH: 3.5; pressure: 1 atm; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - chemical shift assignment
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
NMR spectrometers:
- Varian INOVA 600 MHz