BMRB Entry 15346

Title:
NMR chemical shift assignments for E. coli YejL protein: target ER309 of the Northeast Structural Genomics Consortium
Deposition date:
2007-06-29
Original release date:
2007-07-25
Authors:
Cort, John; Zhao, Li; Jiang, Mei; Cunningham, Kellie; Ma, Li-Chung; Xiao, Rong; Liu, Jinfeng; Baran, Michael; Swapna, G.V.T.; Acton, Thomas; Rost, Burkhard; Montelione, Gaetano; Kennedy, Michael
Citation:

Citation: Cort, John; Montelione, Gaetano; Acton, Thomas; Kennedy, Michael. "NMR Chemical Shift Assignments of E. coli YejL protein"  .

Assembly members:

Assembly members:
YejL, polymer, 83 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts360
15N chemical shifts94
1H chemical shifts562

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1YejL, subunit A1
2YejL, subunit B1

Entities:

Entity 1, YejL, subunit A 83 residues - Formula weight is not available

1   METPROGLNILESERARGTYRSERASPGLU
2   GLNVALGLUGLNLEULEUALAGLULEULEU
3   ASNVALLEUGLULYSHISLYSALAPROTHR
4   ASPLEUSERLEUMETVALLEUGLYASNMET
5   VALTHRASNLEUILEASNTHRSERILEALA
6   PROALAGLNARGGLNALAILEALAASNSER
7   PHEALAARGALALEUGLNSERSERILEASN
8   GLUASPLYSALAHISLEUGLUHISHISHIS
9   HISHISHIS

Samples:

sample_1: YejL, [U-100% 13C; U-100% 15N], 1.5 ± 0.2 mM; D2O 5 ± 0.1 %; sodium chloride 100 mM; ammonium acetate 20 mM; DTT 10 mM; sodium azide 0.02%; calcium chloride 5 mM

sample_2: YejL, [U-100% 13C; U-100% 15N], 1.5 ± 0.2 mM; D2O 100 ± 0.1 %; sodium chloride 100 mM; ammonium acetate 20 mM; DTT 10 mM; sodium azide 0.02%; calcium chloride 5 mM

sample_3: YejL, [5% 13C; U-100% 15N], 1.5 ± .2 mM; D2O 5 ± .1 %; sodium chloride 100 mM; ammonium acetate 20 mM; DTT 10 mM; sodium azide 0.02%; calcium chloride 5 mM

sample_conditions_1: ionic strength: 130 mM; pH: 5.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_3isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
4d 1H-13C-13C-1H HMQC NOESYsample_2isotropicsample_conditions_1
3D 1H-13C edited-filtered NOESYsample_1isotropicsample_conditions_1

Software:

VNMR, Varian - collection

FELIX v97, Accelrys Software Inc. - processing

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 600 MHz
  • Varian INOVA 750 MHz

Related Database Links:

PDB
DBJ BAB36502 BAE76652 BAG77980 BAI26312 BAI31437
EMBL CAP76689 CAQ32593 CAQ99114 CAR03618 CAR08819
GB AAA16383 AAC75248 AAG57325 AAN43793 AAN81179
REF NP_311106 NP_416692 NP_708086 WP_001135663 WP_001135664
SP A7ZP12 A8A251 B1IY82 B1LKT7 B1X885
AlphaFold A7ZP12 A8A251 B1IY82 B1LKT7 B1X885

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks