BMRB Entry 15336
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15336
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Baker, Jennifer; Hudson, Rhea; Kanelis, Voula; Choy, Wing-Yiu; Thibodeau, Patrick; Thomas, Philip; Forman-Kay, Julie. "CFTR regulatory region interacts with NBD1 predominantly via multiple transient helices." Nat. Struct. Mol. Biol. 14, 738-745 (2007).
PubMed: 17660831
Assembly members:
CFTR_regulatory_region, polymer, 189 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pPROEX
Entity Sequences (FASTA):
CFTR_regulatory_region: GAMESAERRNSILTETLHRF
SLEGDAPVSWTETKKQSFKQ
TGEFGEKRKNSILNPINSIR
KFSIVQKTPLQMNGIEEDSD
EPLERRLSLVPDSEQGEAIL
PRISVISTGPTLQARRRQSV
LNLMTHSVNQGQNIHRKTTA
STRKVSLAPQANLTELDIYS
RRLSQETGLEISEEINEEDL
KECLFDDME
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 532 |
| 15N chemical shifts | 176 |
| 1H chemical shifts | 176 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | regulatory region | 1 |
Entities:
Entity 1, regulatory region 189 residues - Formula weight is not available
| 1 | GLY | ALA | MET | GLU | SER | ALA | GLU | ARG | ARG | ASN | ||||
| 2 | SER | ILE | LEU | THR | GLU | THR | LEU | HIS | ARG | PHE | ||||
| 3 | SER | LEU | GLU | GLY | ASP | ALA | PRO | VAL | SER | TRP | ||||
| 4 | THR | GLU | THR | LYS | LYS | GLN | SER | PHE | LYS | GLN | ||||
| 5 | THR | GLY | GLU | PHE | GLY | GLU | LYS | ARG | LYS | ASN | ||||
| 6 | SER | ILE | LEU | ASN | PRO | ILE | ASN | SER | ILE | ARG | ||||
| 7 | LYS | PHE | SER | ILE | VAL | GLN | LYS | THR | PRO | LEU | ||||
| 8 | GLN | MET | ASN | GLY | ILE | GLU | GLU | ASP | SER | ASP | ||||
| 9 | GLU | PRO | LEU | GLU | ARG | ARG | LEU | SER | LEU | VAL | ||||
| 10 | PRO | ASP | SER | GLU | GLN | GLY | GLU | ALA | ILE | LEU | ||||
| 11 | PRO | ARG | ILE | SER | VAL | ILE | SER | THR | GLY | PRO | ||||
| 12 | THR | LEU | GLN | ALA | ARG | ARG | ARG | GLN | SER | VAL | ||||
| 13 | LEU | ASN | LEU | MET | THR | HIS | SER | VAL | ASN | GLN | ||||
| 14 | GLY | GLN | ASN | ILE | HIS | ARG | LYS | THR | THR | ALA | ||||
| 15 | SER | THR | ARG | LYS | VAL | SER | LEU | ALA | PRO | GLN | ||||
| 16 | ALA | ASN | LEU | THR | GLU | LEU | ASP | ILE | TYR | SER | ||||
| 17 | ARG | ARG | LEU | SER | GLN | GLU | THR | GLY | LEU | GLU | ||||
| 18 | ILE | SER | GLU | GLU | ILE | ASN | GLU | GLU | ASP | LEU | ||||
| 19 | LYS | GLU | CYS | LEU | PHE | ASP | ASP | MET | GLU |
Samples:
sample_1: CFTR regulatory region, [U-98% 13C; U-98% 15N], 0.2 mM; potassium chloride 125 mM; potassium phosphate 125 mM; DTT 5 mM; EDTA 5 mM; benzamidine 5 mM; DSS 2 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.25 M; pH: 6.8; pressure: 1 atm; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRView, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Johnson, One Moon Scientific - chemical shift assignment, processing
NMR spectrometers:
- Varian INOVA 800 MHz
Related Database Links:
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks