BMRB Entry 15326

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15326

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Title:
Backbone 13C, HN and 15N assignments for human chemerin
Deposition date:
2007-06-22
Original release date:
2007-09-17
Authors:
Allen, Samantha; Handel, Tracy; Zabel, Brian; Nietlispach, Daniel; Butcher, Eugene; Kirkpatrick, John
Citation:

Citation: Allen, Samantha; Zabel, Brian; Kirkpatrick, John; Butcher, Eugene; Nietlispach, Daniel; Handel, Tracy. "NMR assignment of human chemerin, a novel chemoattractant"  Biomol. NMR Assignments 1, 171-173 (2007).
PubMed: 19636857

Assembly members:

Assembly members:
Chemerin, polymer, 145 residues, 16962 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Chemerin: MELTEAQRRGLQVALEEFHK HPPVQWAFQETSVESAVDTP FPAGIFVRLEFKLQQTSCRK RDWKKPECKVRPNGRKRKCL ACIKLGSEDKVLGRLVHCPI ETQVLREAEEHQETQCLRVQ RAGEDPHSFYFPGQFAPHHH HHHHH

Data sets:
Data typeCount
13C chemical shifts498
15N chemical shifts122
1H chemical shifts122

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1chemerin1

Entities:

Entity 1, chemerin 145 residues - 16962 Da.

Residue 1 is a non-native initiator met. The last 9 residues are also non-native (a proline and His8 tag).

1   METGLULEUTHRGLUALAGLNARGARGGLY
2   LEUGLNVALALALEUGLUGLUPHEHISLYS
3   HISPROPROVALGLNTRPALAPHEGLNGLU
4   THRSERVALGLUSERALAVALASPTHRPRO
5   PHEPROALAGLYILEPHEVALARGLEUGLU
6   PHELYSLEUGLNGLNTHRSERCYSARGLYS
7   ARGASPTRPLYSLYSPROGLUCYSLYSVAL
8   ARGPROASNGLYARGLYSARGLYSCYSLEU
9   ALACYSILELYSLEUGLYSERGLUASPLYS
10   VALLEUGLYARGLEUVALHISCYSPROILE
11   GLUTHRGLNVALLEUARGGLUALAGLUGLU
12   HISGLNGLUTHRGLNCYSLEUARGVALGLN
13   ARGALAGLYGLUASPPROHISSERPHETYR
14   PHEPROGLYGLNPHEALAPROHISHISHIS
15   HISHISHISHISHIS

Samples:

13C_15N_chemerin: Chemerin, [U-98% 13C; U-98% 15N], 0.8 – 1.5 mM; acetic acid, [U-2H], 10 mM; EDTA, [U-2H], 0.1 mM

15N_chemerin: Chemerin, [U-98% 15N], 0.8 mM; EDTA, [U-2H], 0.1 mM; acetic acid, [U-2H], 10 mM

sample_conditions_1: pH: 5.49; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC15N_chemerinisotropicsample_conditions_1
3D CBCA(CO)NH13C_15N_chemerinisotropicsample_conditions_1
3D C(CO)NH13C_15N_chemerinisotropicsample_conditions_1
3D HNCO13C_15N_chemerinisotropicsample_conditions_1
CBCANH13C_15N_chemerinisotropicsample_conditions_1

Software:

AZARA v2.7, Boucher - processing

Analysis v1.0.14, CCPN - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

DBJ BAA76499 BAG35130
EMBL CAG46789 CAG46823 CAH93115
GB AAB47975 AAH00069 AAS00384 AAX36662 AAX37088
REF NP_001127631 NP_002880 XP_001134678 XP_003815095 XP_004046507
SP Q5R551 Q99969
AlphaFold Q5R551 Q99969

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks