BMRB Entry 15322

Name: 1H, 13C, and 15N Chemical Shift Assignments for Chemotaxis Protein CheW
Published: 2008-02-29

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15322

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N Chemical Shift Assignments for Chemotaxis Protein CheW

Deposition date:

2007-06-21

Original release date:

2008-02-29

Authors:

Jin, Changwen; Li, You; Fu, Wenyu

Citation:

Citation: Li, You; Hu, Yunfei; Fu, Wenyu; Xia, Bin; Jin, Changwen. "Solution structure of the bacterial chemotaxis adaptor protein CheW from Escherichia coli" Biochem. Biophys. Res. Commun. 360, 863-867 (2007).
PubMed: 17631272

Assembly members:

Assembly members:
chemotaxis_protein_CheW, polymer, 167 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
chemotaxis_protein_CheW: MTGMTNVTKLASEPSGQEFL VFTLGDEEYGIDILKVQEIR GYDQVTRIANTPAFIKGVTN LRGVIVPIVDLRIKFSQVDV DYNDNTVVIVLNLGQRVVGI VVDGVSDVLSLTAEQIRPAP EFAVTLSTEYLTGLGALGDR MLILVNIEKLLNSEEMALLD SAASEVA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	669
15N chemical shifts	168
1H chemical shifts	1146

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	monomer	1

Entities:

Entity 1, monomer 167 residues - Formula weight is not available

1

MET

THR

GLY

MET

THR

ASN

VAL

THR

LYS

LEU

2

ALA

SER

GLU

PRO

SER

GLY

GLN

GLU

PHE

LEU

3

VAL

PHE

THR

LEU

GLY

ASP

GLU

TYR

GLY

4

ILE

ASP

ILE

LEU

LYS

VAL

GLN

GLU

ILE

ARG

5

GLY

TYR

ASP

GLN

VAL

THR

ARG

ILE

ALA

ASN

6

THR

PRO

ALA

PHE

ILE

LYS

GLY

VAL

THR

ASN

7

LEU

ARG

GLY

VAL

ILE

VAL

PRO

ILE

VAL

ASP

8

LEU

ARG

ILE

LYS

PHE

SER

GLN

VAL

ASP

VAL

9

ASP

TYR

ASN

ASP

ASN

THR

VAL

ILE

VAL

10

LEU

ASN

LEU

GLY

GLN

ARG

VAL

GLY

ILE

11

VAL

ASP

GLY

VAL

SER

ASP

VAL

LEU

SER

12

LEU

THR

ALA

GLU

GLN

ILE

ARG

PRO

ALA

PRO

13

GLU

PHE

ALA

VAL

THR

LEU

SER

THR

GLU

TYR

14

LEU

THR

GLY

LEU

GLY

ALA

LEU

GLY

ASP

ARG

15

MET

LEU

ILE

LEU

VAL

ASN

ILE

GLU

LYS

LEU

16

LEU

ASN

SER

GLU

MET

ALA

LEU

ASP

17

SER

ALA

SER

GLU

VAL

ALA

Samples:

sample_1: chemotaxis protein CheW, [U-95% 13C; U-95% 15N], 1 mM; Tris-HCl 30 mM; NaCl 30 mM

sample_conditions_1: ionic strength: 0.06 M; pH: 7.3; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

Bruker Avance 500 MHz

PDB	2HO9
DBJ	BAA15703 BAB36020 BAG77646 BAI25978 BAI30941
EMBL	CAP76375 CAQ32364 CAQ98827 CAR03246 CAR08346
GB	AAA23565 AAC74957 AAG56877 AAN43489 AAN80761
PIR	A85802
REF	NP_310624 NP_416401 NP_707782 WP_000147297 WP_000147298
SP	P0A964 P0A965 P0A966 P0A967
AlphaFold	P0A964 P0A965 P0A966 P0A967