BMRB Entry 15309
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15309
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Citation: Tjong, Siu-Cin; Wu, Po-Long; Wang, Chang-Mao; Huang, Wei-Ning; Ho, Nan-Lu; Wu, Wen-Guey. "Role of glycosphingolipid conformational change in membrane pore forming activity of cobra cardiotoxin" Biochemistry 46, 12111-12123 (2007).
PubMed: 17918958
Assembly members:
CTX_A5, polymer, 62 residues, Formula weight is not available
Natural source: Common Name: Chinese cobra Taxonomy ID: 8656 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Naja atra
Experimental source: Production method: purified from the natural source
Entity Sequences (FASTA):
CTX_A5: LKCHNTQLPFIYKTCPEGKN
LCFKATLKKFPLKFPVKRGC
ADNCPKNSALLKYVCCSTDK
CN
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 1073 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | cardiotoxin A5 | 1 |
Entities:
Entity 1, cardiotoxin A5 62 residues - Formula weight is not available
| 1 | LEU | LYS | CYS | HIS | ASN | THR | GLN | LEU | PRO | PHE | ||||
| 2 | ILE | TYR | LYS | THR | CYS | PRO | GLU | GLY | LYS | ASN | ||||
| 3 | LEU | CYS | PHE | LYS | ALA | THR | LEU | LYS | LYS | PHE | ||||
| 4 | PRO | LEU | LYS | PHE | PRO | VAL | LYS | ARG | GLY | CYS | ||||
| 5 | ALA | ASP | ASN | CYS | PRO | LYS | ASN | SER | ALA | LEU | ||||
| 6 | LEU | LYS | TYR | VAL | CYS | CYS | SER | THR | ASP | LYS | ||||
| 7 | CYS | ASN |
Samples:
CTX_A5_sample1: CTX A5 1 mM; phosphate buffer 10 mM
CTX_A5_in_DPC_micelle: CTX A5 1 mM; DPC, [U-2H], 50 mM; phosphate buffer 10 mM
CTX_A5_in_10%SGC_DPC_micelle: CTX A5 1 mM; SGC 5 mM; DPC, [U-2H], 45 mM; phosphate buffer 10 mM
sample_conditions_1: ionic strength: 10 mM; pH: 6.0; pressure: 1 atm; temperature: 318 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | CTX_A5_sample1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | CTX_A5_sample1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | CTX_A5_in_DPC_micelle | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | CTX_A5_in_10%SGC_DPC_micelle | isotropic | sample_conditions_1 |
Software:
xwinnmr, Bruker Biospin - chemical shift assignment
NMR spectrometers:
- Bruker DMX 500 MHz