BMRB Entry 15289

Name: 1H, 15N chemical shift assignment of the THAP domain 1-81 from human THAP1 protein
Published: 2008-02-21

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR15289

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N chemical shift assignment of the THAP domain 1-81 from human THAP1 protein

Deposition date:

2007-06-06

Original release date:

2008-02-21

Authors:

BESSIERE, Damien; CAMPAGNE, Sebastien; MILON, Alain; GERVAIS, Virginie

Citation:

Citation: Bessiere, Damien; Lacroix, Chrystelle; Campagne, Sebastien; Ecochard, Vincent; Guillet, Valerie; Mourey, Lionel; Lopez, Frederic; Czaplicki, J.; Demange, Pascal; Milon, Alain; Cirard, Jean-Philippe; Gervais, Virginie. "Structure-Function Analysis of the THAP Zinc Finger of THAP1, a Large C2CH DNA-binding Module Linked to Rb/E2F Pathways." J. Biol. Chem. 283, 4352-4363 (2008).
PubMed: 18073205

Assembly members:

Assembly members:
THAP_domain, polymer, 87 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet26bII

Entity Sequences (FASTA):

Entity Sequences (FASTA):
THAP_domain: MVQSCSAYGCKNRYDKDKPV SFHKFPLTRPSLCKEWEAAV RRKNFKPTKYSSICSEHFTP DCFKRECNNKLLKENAVPTI FLELVPR

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1H

Data type	Count
15N chemical shifts	76
1H chemical shifts	565

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	THAP domain polypeptide	1
2	ZINC ION	2

Entities:

Entity 1, THAP domain polypeptide 87 residues - Formula weight is not available

1

MET

VAL

GLN

SER

CYS

SER

ALA

TYR

GLY

CYS

2

LYS

ASN

ARG

TYR

ASP

LYS

ASP

LYS

PRO

VAL

3

SER

PHE

HIS

LYS

PHE

PRO

LEU

THR

ARG

PRO

4

SER

LEU

CYS

LYS

GLU

TRP

GLU

ALA

VAL

5

ARG

LYS

ASN

PHE

LYS

PRO

THR

LYS

TYR

6

SER

ILE

CYS

SER

GLU

HIS

PHE

THR

PRO

7

ASP

CYS

PHE

LYS

ARG

GLU

CYS

ASN

LYS

8

LEU

LYS

GLU

ASN

ALA

VAL

PRO

THR

ILE

9

PHE

LEU

GLU

LEU

VAL

PRO

ARG

Entity 2, ZINC ION - Zn - 65.409 Da.

1

ZN

Samples:

sample_U: THAP domain 1 mM; NaCl 10 mM; Tris 50 mM; DTT 1 mM

sample_U-15N: THAP domain, [U-15N], 1 mM; NaCl 10 mM; Tris 50 mM; DTT 1 mM

sample_conditions: ionic strength: 10 mM; pH: 6.8; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_U	isotropic	sample_conditions
2D 1H-1H TOCSY	sample_U	isotropic	sample_conditions
2D 1H-1H COSY	sample_U	isotropic	sample_conditions
2D 1H-15N HSQC	sample_U-15N	isotropic	sample_conditions
3D 1H-15N NOESY	sample_U-15N	isotropic	sample_conditions
3D 1H-15N TOCSY	sample_U-15N	isotropic	sample_conditions

Software:

XEASY, Bartels et al. - chemical shift assignment

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data processing

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 700 MHz
Bruker Avance 800 MHz

BMRB	16485
PDB	2JTG 2KO0 2L1G
DBJ	BAA91635 BAD96951 BAE02260 BAI45652
EMBL	CAG33537 CAH90563
GB	AAH21721 AAI02410 ADZ15806 AIC51723 AKI69887
REF	NP_001029820 NP_001125299 NP_001244808 NP_001271632 NP_060575
SP	Q3T0G1 Q4R3Q6 Q5RCE4 Q9NVV9
TPG	DAA14452
AlphaFold	Q3T0G1 Q4R3Q6 Q5RCE4 Q9NVV9