BMRB Entry 15282

Title:
Backbone 1H Chemical Shift Assignments for N-Me-Phe5
Deposition date:
2007-06-05
Original release date:
2009-02-06
Authors:
Sabo, Jennifer; Harris, Karen; Coley, Andrew; Karas, John; Casey, Joanne; Tan, Yen Yee; Norton, Raymond; Hughes, Andrew; Scanlon, Denis; Foley, Michael
Citation:

Citation: Harris, Karen; Casey, Joanne; Coley, Andrew; Karas, John; Sabo, Jennifer; Tan, Yen Yee; Dolezal, Olan; Norton, Raymond; Hughes, Andrew; Scanlon, Denis; Foley, Michael. "Rapid optimization of a peptide inhibitor of malaria parasite invasion by comprehensive N-methyl scanning."  J. Biol. Chem. 284, 9361-9371 (2009).
PubMed: 19164290

Assembly members:

Assembly members:
N-Me-Phe5, polymer, 20 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
N-Me-Phe5: VFAEXLPLFSKFGSRMHILK

Data sets:
Data typeCount
1H chemical shifts130

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1N-Me-Phe51

Entities:

Entity 1, N-Me-Phe5 20 residues - Formula weight is not available

Methylation of backbone amide for Phe5

1   VALPHEALAGLUMEALEUPROLEUPHESER
2   LYSPHEGLYSERARGMETHISILELEULYS

Samples:

sample_1: N-Me-Phe5 1.7 mM; H2O 95%; D2O 5%

sample_conditions_1: pH: 4.6; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v1.3, Bruker Biospin - collection, processing

NMR spectrometers:

  • Bruker DRX 600 MHz