BMRB Entry 15280

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15280

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Title:
1H, 13C and 15N resonance assignment of the oxidized form (Cys67-Cys70) of the N-terminal domain of PilB from Neisseria meningitidis
Deposition date:
2007-06-05
Original release date:
2007-07-18
Authors:
Quinternet, Marc; Beaufils, Chrystel; Neiers, Fabrice; Tsan, Pascale; Boschi-Muller, Sandrine; Averlant-petit, Marie-Christine; Branlant, Guy; Cung, Manh-Thong
Citation:

Citation: Quinternet, Marc; Beaufils, Chrystel; Neiers, Fabrice; Tsan, Pascale; Boschi-Muller, Sandrine; Averlant-Petit, Marie-Christine; Branlant, Guy; Cung, Manh-Thong. "1H, 13C and 15N resonance assignment of the oxidized form (Cys(67)-Cys (70)) of the N-terminal domain of PilB from Neisseria meningitidis"  Biomol. NMR Assignments 1, 143-145 (2007).
PubMed: 19636850

Assembly members:

Assembly members:
Dsbe_ox, polymer, 144 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Neisseria meningitidis   Taxonomy ID: 487   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Neisseria meningitidis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Dsbe_ox: MVPHTLSTLKTADNRPASVY LKKDKPTLIKFWASWCPLCL SELGQTEKWAQDAKFSSANL ITVASPGFLHEKKDGDFQKW YAGLNYPKLPVVTDNGGTIA QSLNISVYPSWALIGKDGDV QRIVKGSINEAQALALIRDP NADL

Data sets:
Data typeCount
13C chemical shifts541
15N chemical shifts134
1H chemical shifts854

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Dsbe_ox1

Entities:

Entity 1, Dsbe_ox 144 residues - Formula weight is not available

1   METVALPROHISTHRLEUSERTHRLEULYS
2   THRALAASPASNARGPROALASERVALTYR
3   LEULYSLYSASPLYSPROTHRLEUILELYS
4   PHETRPALASERTRPCYSPROLEUCYSLEU
5   SERGLULEUGLYGLNTHRGLULYSTRPALA
6   GLNASPALALYSPHESERSERALAASNLEU
7   ILETHRVALALASERPROGLYPHELEUHIS
8   GLULYSLYSASPGLYASPPHEGLNLYSTRP
9   TYRALAGLYLEUASNTYRPROLYSLEUPRO
10   VALVALTHRASPASNGLYGLYTHRILEALA
11   GLNSERLEUASNILESERVALTYRPROSER
12   TRPALALEUILEGLYLYSASPGLYASPVAL
13   GLNARGILEVALLYSGLYSERILEASNGLU
14   ALAGLNALALEUALALEUILEARGASPPRO
15   ASNALAASPLEU

Samples:

sample_1: potassium phosphate, [U-13C; U-15N], 50 mM

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HNHBsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment

XEASY, Bartels et al. - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
EMBL CAA32146 CAM07595 CAM09346 CAX49031 CBA03711
GB AAB97511 AAF40515 AAL89752 AAW90666 ABX72275
REF NP_273110 WP_002216163 WP_002218473 WP_002221795 WP_002223263
SP P14930 Q9JWM8 Q9K1N8
AlphaFold P14930 Q9JWM8 Q9K1N8

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks