BMRB Entry 15276

Name: 1H, 13C and 15N resonance assignment of 6aJL2(R25G), a highly fibrillogenic lamdaVI light chain variable domain.
Published: 2007-09-17

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15276

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N resonance assignment of 6aJL2(R25G), a highly fibrillogenic lamdaVI light chain variable domain.

Deposition date:

2007-06-01

Original release date:

2007-09-17

Authors:

Gutierrez-Gonzalez, Luis; Muresanu, Lucia; del Pozo-Yauner, Luis; Sanchez, Rosalba; Guereca, Leopoldo; Becerril, Baltazar; Luecke, Christian

Citation:

Citation: Gutierrez-Gonzalez, Luis; Muresanu, Lucia; del Pozo-Yauner, Luis; Sanchez, Rosalba; Guereca, Leopoldo; Becerril, Baltazar; Lucke, Christian. "1H, 13C and 15N resonance assignment of 6aJL2(R25G), a highly fibrillogenic lamdaVI light chain variable domain." Biomol. NMR Assignments 1, 159-161 (2007).
PubMed: 19636854

Assembly members:

Assembly members:
6aJL2, polymer, 111 residues, 11853.8 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET27b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
6aJL2: NFMLTQPHSVSESPGKTVTI SCTGSSGSIASNYVQWYQQR PGSSPTTVIYEDNQRPSGVP DRFSGSIDSSSNSASLTISG LKTEDEADYYCQSYDSSNHV VFGGGTKLTVL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	464
15N chemical shifts	119
1H chemical shifts	713

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	6aJL2	1

Entities:

Entity 1, 6aJL2 111 residues - 11853.8 Da.

Residue number 20 does not exist in this sequence numbering

1

ASN

PHE

MET

LEU

THR

GLN

PRO

HIS

SER

VAL

2

SER

GLU

SER

PRO

GLY

LYS

THR

VAL

THR

ILE

3

SER

CYS

THR

GLY

SER

GLY

SER

ILE

ALA

4

SER

ASN

TYR

VAL

GLN

TRP

TYR

GLN

ARG

5

PRO

GLY

SER

PRO

THR

VAL

ILE

TYR

6

GLU

ASP

ASN

GLN

ARG

PRO

SER

GLY

VAL

PRO

7

ASP

ARG

PHE

SER

GLY

SER

ILE

ASP

SER

8

SER

ASN

SER

ALA

SER

LEU

THR

ILE

SER

GLY

9

LEU

LYS

THR

GLU

ASP

GLU

ALA

ASP

TYR

10

CYS

GLN

SER

TYR

ASP

SER

ASN

HIS

VAL

11

VAL

PHE

GLY

THR

LYS

LEU

THR

VAL

12

LEU

Samples:

sample_1: 6aJL2 1.2 mM; sodium phosphate 25 mM; sodium azide 0.05%; H2O 95%; D2O 5%

sample_2: 6aJL2, [U-95% 15N], 1.2 mM; sodium phosphate 25 mM; sodium azide 0.05%; H2O 95%; D2O 5%

sample_3: 6aJL2, [U-95% 13C; U-95% 15N], 1.2 mM; sodium phosphate 25 mM; sodium azide 0.05%; H2O 95%; D2O 5%

sample_4: 6aJL2 1.3 mM; sodium phosphate 25 mM; sodium azide 0.05%; D2O 100%

sample_conditions_1: ionic strength: 25 mM; pH: 5.2; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-15N HTQC	sample_2	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_2	isotropic	sample_conditions_1
3D HNCA	sample_3	isotropic	sample_conditions_1
3D HNCO	sample_3	isotropic	sample_conditions_1
3D HNCACB	sample_3	isotropic	sample_conditions_1
3D HNCACO	sample_3	isotropic	sample_conditions_1
3D C(CO)NH	sample_3	isotropic	sample_conditions_1
3D H(CCO)NH	sample_3	isotropic	sample_conditions_1
3D H(C)CH-TOCSY	sample_3	isotropic	sample_conditions_1
3D (H)CCH-TOCSY	sample_3	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_3	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_3	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_4	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_4	isotropic	sample_conditions_1

Software:

xwinnmr v3.5, Bruker Biospin - collection

AURELIA v3.1.6, Neidig, Geyer, Gorler, Antz, Saffrich, Beneicke, Kalbitzer - data analysis

FELIX v2000, Accelrys Software Inc. - data analysis

NMR spectrometers:

Bruker DRX 500 MHz

BMRB	19798 19870
PDB	2MKW 2MMX 2W0K 2W0L 3B5G 3BDX 5C9K
DBJ	BAA19991 BAC01822 BAC01823 BAC01824 BAC01857
EMBL	CAD43014 CAJ75494 CAJ75495 CAP74492 CAP74493
GB	AAB33217 AAG24687 AAG24691 AAG47681 AAM46210