BMRB Entry 15266

Name: 1H, 13C, and 15N Backbone Resonance Assignments of the Carboxyl Terminal Domain of Connexin40
Published: 2007-09-17

Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15266

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

1H, 13C, and 15N Backbone Resonance Assignments of the Carboxyl Terminal Domain of Connexin40

Deposition date:

2007-05-24

Original release date:

2007-09-17

Authors:

Bouvier, Denis; Kieken, Fabien

Citation:

Citation: Bouvier, Denis; Kieken, Fabien; Sorgen, Paul. "(1)H, (13)C, and (15)N backbone resonance assignments of the carboxyl terminal domain of Connexin40" Biomol. NMR Assignments 1, 155-157 (2007).
PubMed: 19636853

Assembly members:

Assembly members:
Connexin43, polymer, 109 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-6P-2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Connexin43: GPLGSTSLVQGLTPPPDFNQ CLKNSPDEKFFSDFSNNMGS RKNPDPLATEEVPNQEQIPE EGFIHTQYGQKPEQPSGASA GHRFPQGYHSDKRRLSKASS KARSDDLSV

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	418
15N chemical shifts	98
1H chemical shifts	692

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Connexin40	1

Entities:

Entity 1, Connexin40 109 residues - Formula weight is not available

1

GLY

PRO

LEU

GLY

SER

THR

SER

LEU

VAL

GLN

2

GLY

LEU

THR

PRO

ASP

PHE

ASN

GLN

3

CYS

LEU

LYS

ASN

SER

PRO

ASP

GLU

LYS

PHE

4

PHE

SER

ASP

PHE

SER

ASN

MET

GLY

SER

5

ARG

LYS

ASN

PRO

ASP

PRO

LEU

ALA

THR

GLU

6

GLU

VAL

PRO

ASN

GLN

GLU

GLN

ILE

PRO

GLU

7

GLU

GLY

PHE

ILE

HIS

THR

GLN

TYR

GLY

GLN

8

LYS

PRO

GLU

GLN

PRO

SER

GLY

ALA

SER

ALA

9

GLY

HIS

ARG

PHE

PRO

GLN

GLY

TYR

HIS

SER

10

ASP

LYS

ARG

LEU

SER

LYS

ALA

SER

11

LYS

ALA

ARG

SER

ASP

LEU

SER

VAL

Samples:

sample_1: Connexin40, [U-98% 13C; U-98% 15N], 1 mM; Phosphate 11.9 mM; Sodium Chloride 137 mM; Potassium Chloride 2.7 mM

sample_conditions_1: ionic strength: 150 mM; pH: 6.0; pressure: 1 atm; temperature: 280 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Varian INOVA 600 MHz

PDB	2K7M
GB	AAA41000 AAA41194 AAC53502 AAC53503 AAH70935
REF	NP_062153 XP_008759550
SP	P28234
AlphaFold	P28234