BMRB Entry 15250

Name: Main chain NMR assignments of SBT70
Published: 2008-01-28

Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15250

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Main chain NMR assignments of SBT70

Deposition date:

2007-05-16

Original release date:

2008-01-28

Authors:

Sari, Nese; Bryan, Philip; Orban, John

Citation:

Citation: Sari, Nese; Fisher, Kathryn; Bryan, Philip; Orban, John. "Main chain NMR assignments of subtilisin Sbt70 in its prodomain-bound state" Biomol. NMR Assignments 1, 209-211 (2007).
PubMed: 19636867

Assembly members:

Assembly members:
Sbt70, polymer, 266 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pj12

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Sbt70: AQSVPYGVSQIKAPALHSQG YTGSNVKVAVIDSGIDSSHP DLNVAGGASFVPSETNPFQD NNSHGTHVAGTVLAVAPSAS LYAVKVLGADGSGQYSWIIN GIEWAIANNMDVINMSLGGP SGSAALKAAVDKAVASGVVV VAAAGNEGTSGSSSTVGYPG KYPSVIAVGAVDSSNQRASF SSVGPELDVMAPGVSIVSTL PGNKYGAKSGTAMASPHVAG AAALILSKHPNWTNTQVRSS LENTTTKLGDSFYYGKGLIN VEAAAQ

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	594
15N chemical shifts	193
1H chemical shifts	193

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Sbt70	1

Entities:

Entity 1, Sbt70 266 residues - Formula weight is not available

1

ALA

GLN

SER

VAL

PRO

TYR

GLY

VAL

SER

GLN

2

ILE

LYS

ALA

PRO

ALA

LEU

HIS

SER

GLN

GLY

3

TYR

THR

GLY

SER

ASN

VAL

LYS

VAL

ALA

VAL

4

ILE

ASP

SER

GLY

ILE

ASP

SER

HIS

PRO

5

ASP

LEU

ASN

VAL

ALA

GLY

ALA

SER

PHE

6

VAL

PRO

SER

GLU

THR

ASN

PRO

PHE

GLN

ASP

7

ASN

SER

HIS

GLY

THR

HIS

VAL

ALA

GLY

8

THR

VAL

LEU

ALA

VAL

ALA

PRO

SER

ALA

SER

9

LEU

TYR

ALA

VAL

LYS

VAL

LEU

GLY

ALA

ASP

10

GLY

SER

GLY

GLN

TYR

SER

TRP

ILE

ASN

11

GLY

ILE

GLU

TRP

ALA

ILE

ALA

ASN

MET

12

ASP

VAL

ILE

ASN

MET

SER

LEU

GLY

PRO

13

SER

GLY

SER

ALA

LEU

LYS

ALA

VAL

14

ASP

LYS

ALA

VAL

ALA

SER

GLY

VAL

15

VAL

ALA

GLY

ASN

GLU

GLY

THR

SER

16

GLY

SER

THR

VAL

GLY

TYR

PRO

GLY

17

LYS

TYR

PRO

SER

VAL

ILE

ALA

VAL

GLY

ALA

18

VAL

ASP

SER

ASN

GLN

ARG

ALA

SER

PHE

19

SER

VAL

GLY

PRO

GLU

LEU

ASP

VAL

MET

20

ALA

PRO

GLY

VAL

SER

ILE

VAL

SER

THR

LEU

21

PRO

GLY

ASN

LYS

TYR

GLY

ALA

LYS

SER

GLY

22

THR

ALA

MET

ALA

SER

PRO

HIS

VAL

ALA

GLY

23

ALA

LEU

ILE

LEU

SER

LYS

HIS

PRO

24

ASN

TRP

THR

ASN

THR

GLN

VAL

ARG

SER

25

LEU

GLU

ASN

THR

LYS

LEU

GLY

ASP

26

SER

PHE

TYR

GLY

LYS

GLY

LEU

ILE

ASN

27

VAL

GLU

ALA

GLN

Samples:

sample_1: Sbt70, [U-95% 13C; U-95% 15N], 0.7 mM; KPi 0.1 M; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 7.2; pressure: 1 atm; temperature: 318 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe v2.3, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY v3.110, Goddard - chemical shift assignment, data analysis

NMR spectrometers:

Bruker DRX 600 MHz
Bruker Avance 600 MHz

BMRB	15248
PDB	1DUI 1GNS 1GNV 1SPB 1SUA 1SUE