BMRB Entry 15247

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15247
MolProbity Validation Chart

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Title:
1H, 13C and 15N resonance assignments of the Escherichia coli YaeT POTRA domain
Deposition date:
2007-05-14
Original release date:
2007-08-22
Authors:
Knowles, Timothy; Bobat, Saeeda; Jeeves, Mark; Henderson, Ian; Overduin, Michael
Citation:

Citation: Knowles, Timothy; Bobat, Saeeda; Jeeves, Mark; Henderson, Ian; Overduin, Michael. "Secondary structure and 1H, 13C and 15N resonance assignments of the Escherichia coli YaeT POTRA domain"  Biomol. NMR Assignments 1, 113-115 (2007).
PubMed: 19636842

Assembly members:

Assembly members:
POTRA, polymer, 164 residues, 17967.2 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQETCH

Entity Sequences (FASTA):

Entity Sequences (FASTA):
POTRA: AEGFVVKDIHFEGLQRVAVG AALLSMPVRTGDTVNDEDIS NTIRALFATGNFEDVRVLRD GDTLLVQVKERPTIASITFS GNKSVKDDMLKQNLEASGVR VGESLDRTTIADIEKGLEDF YYSVGKYSASVKAVVTPLPR NRVDLKLVFQEGVSLQHHHH QTAG

Data sets:
Data typeCount
13C chemical shifts669
15N chemical shifts159
1H chemical shifts1078

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1POTRA1

Entities:

Entity 1, POTRA 164 residues - 17967.2 Da.

Residues 1-156 represent the first two POTRA domains of YaeT. Residues 157-164 non-native affinity tag

1   ALAGLUGLYPHEVALVALLYSASPILEHIS
2   PHEGLUGLYLEUGLNARGVALALAVALGLY
3   ALAALALEULEUSERMETPROVALARGTHR
4   GLYASPTHRVALASNASPGLUASPILESER
5   ASNTHRILEARGALALEUPHEALATHRGLY
6   ASNPHEGLUASPVALARGVALLEUARGASP
7   GLYASPTHRLEULEUVALGLNVALLYSGLU
8   ARGPROTHRILEALASERILETHRPHESER
9   GLYASNLYSSERVALLYSASPASPMETLEU
10   LYSGLNASNLEUGLUALASERGLYVALARG
11   VALGLYGLUSERLEUASPARGTHRTHRILE
12   ALAASPILEGLULYSGLYLEUGLUASPPHE
13   TYRTYRSERVALGLYLYSTYRSERALASER
14   VALLYSALAVALVALTHRPROLEUPROARG
15   ASNARGVALASPLEULYSLEUVALPHEGLN
16   GLUGLYVALSERLEUGLNHISHISHISHIS
17   GLNTHRALAGLY

Samples:

sample_1: POTRA, [U-99% 13C; U-99% 15N], 0.8 mM; MES 50 mM; sodium chloride 50 mM; sodium azide 3 mM; Complete protease inhibitor cocktail 1 tablet/100ml

sample_conditions_1: ionic strength: 86 mM; pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D H(C)CH-TOCSYsample_1isotropicsample_conditions_1
3D (H)CCH-TOCSYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1

Software:

SPARKY v3.110, Goddard - chemical shift assignment

TALOS v2003.027.13.05, Cornilescu, Delaglio and Bax - data analysis

NMRPipe v2004.194.17.2, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAA77852 BAB33602 BAG75700 BAI23538 BAI29053
EMBL CAP74746 CAQ30691 CAQ87780 CAQ97064 CAR01552
GB AAB08606 AAC73288 AAD23568 AAG54479 AAK64508
REF NP_308206 NP_414719 NP_706122 WP_001240878 WP_001240879
SP A1A7M1 A7ZHR7 A7ZWC3 A8ALB1 A9MPI4
AlphaFold A1A7M1 A7ZHR7 A7ZWC3 A8ALB1 A9MPI4

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks