BMRB Entry 15236

Name: 1H, 15N backbone chemical shift assignemnt of the G26K,D27S,D31A triple mutant of the protein CyaY
Published: 2007-05-18

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15236

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N backbone chemical shift assignemnt of the G26K,D27S,D31A triple mutant of the protein CyaY

Deposition date:

2007-05-04

Original release date:

2007-05-18

Authors:

Pastore, Chiara; Pastore, Annalisa; Correia, Ana; Gomes, Claudio

Citation:

Citation: Correia, Ana; Pastore, Chiara; Adinolfi, Salvatore; Pastore, Annalisa; Gomes, Claudio. "Dynamics, stability and iron-binding activity of frataxin clinical mutants." FEBS J. 275, 3680-3690 (2008).
PubMed: 18537827

Assembly members:

Assembly members:
CyaYG26KD27SD31K, polymer, 108 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CyaYG26KD27SD31K: GAMNDSEFHRLADQLWLTIE ERLDDWDKSSDIACEINGGV LTITFENGSKIIINRQEPLH QVWLATKQGGYHFDLKGDEW ICDRSGETFWDLLEQAATQQ AGETVSFR

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	104
1H chemical shifts	104

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CyaYG26KD27SD31K	1

Entities:

Entity 1, CyaYG26KD27SD31K 108 residues - Formula weight is not available

1

GLY

ALA

MET

ASN

ASP

SER

GLU

PHE

HIS

ARG

2

LEU

ALA

ASP

GLN

LEU

TRP

LEU

THR

ILE

GLU

3

GLU

ARG

LEU

ASP

TRP

ASP

LYS

SER

4

ASP

ILE

ALA

CYS

GLU

ILE

ASN

GLY

VAL

5

LEU

THR

ILE

THR

PHE

GLU

ASN

GLY

SER

LYS

6

ILE

ASN

ARG

GLN

GLU

PRO

LEU

HIS

7

GLN

VAL

TRP

LEU

ALA

THR

LYS

GLN

GLY

8

TYR

HIS

PHE

ASP

LEU

LYS

GLY

ASP

GLU

TRP

9

ILE

CYS

ASP

ARG

SER

GLY

GLU

THR

PHE

TRP

10

ASP

LEU

GLU

GLN

ALA

THR

GLN

11

ALA

GLY

GLU

THR

VAL

SER

PHE

ARG

Samples:

sample_1: CyaYG26KD27SD31K, [U-98% 15N], 0.4 mM; NaP 20 mM; NaCl 50 mM; DTT 2 mM

sample_conditions_1: pH: 7.0; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

XEASY, Bartels et al. - chemical shift assignment

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Varian INOVA 800 MHz

BMRB	15237
PDB	1EW4 1SOY 2EFF 2P1X
DBJ	BAE77494 BAG79612 BAI27941 BAI33064 BAI38380
EMBL	CAA47281 CAR00776 CAR15464 CAV00925 CBJ03590
GB	AAA67603 AAC76810 AAZ90503 ABB63872 ABV17629
REF	NP_418251 WP_000999947 WP_032267531 WP_042109681 WP_042113898
SP	A7ZU09 B1IW97 B1XAH3 B6I4E2 B7L963
AlphaFold	A7ZU09 B1IW97 B1XAH3 B6I4E2 B7L963