BMRB Entry 15231

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15231

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Title:
ErbB2TM
Deposition date:
2007-05-02
Original release date:
2008-02-20
Authors:
Mineev, Konstantin; Bocharov, Eduard; Mineeva, Eugenia; Volynsky, Pavel; Arseniev, Alexander
Citation:

Citation: Bocharov, Eduard; Mineev, Konstantin; Volynsky, Pavel; Ermolyuk, Y.; Tkach, E.; Sobol, A.; Chupin, V.; Kirpichnikov, M.; Efremov, R.; Arseniev, Alexander. "Spatial structure of the dimeric transmembrane domain of the growth factor receptor ErbB2 presumably corresponding to the receptor active state"  J. Biol. Chem. 283, 6950-6956 (2008).
PubMed: 18178548

Assembly members:

Assembly members:
ErbB2, polymer, 44 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: none

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ErbB2: GCPAEQRASPLTSIISAVVG ILLVVVLGVVFGILIKRRQQ KIRK

Data sets:
Data typeCount
13C chemical shifts160
15N chemical shifts40
1H chemical shifts333
coupling constants38

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ErbB2 chain A1
2ErbB2 chain B1

Entities:

Entity 1, ErbB2 chain A 44 residues - Formula weight is not available

1   GLYCYSPROALAGLUGLNARGALASERPRO
2   LEUTHRSERILEILESERALAVALVALGLY
3   ILELEULEUVALVALVALLEUGLYVALVAL
4   PHEGLYILELEUILELYSARGARGGLNGLN
5   LYSILEARGLYS

Samples:

sample_1: ErbB2, [U-15N], 2.0 mM; DHPC, [U-2H], 64 mM; DMPC, [U-2H], 16 mM; D2O 5%; H2O 95%

sample_2: ErbB2, [U-13C; U-15N], 2.0 mM; DHPC, [U-2H], 64 mM; DMPC, [U-2H], 16 mM; D2O 5%; H2O 95%

heterodimer: ErbB2, [U-13C; U-15N], 1.0 mM; DHPC, [U-2H], 64 mM; DMPC, [U-2H], 16 mM; ErbB2 1.0 mM; D2O 5%; H2O 95%

sample_conditions_1: pH: 5.0; pressure: 1 atm; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HNHBsample_1isotropicsample_conditions_1
3D-C13-CHIRP-NOESYheterodimerisotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

CARA v1.8.3, Wutrich, Keller - chemical shift assignment, data analysis

NMR spectrometers:

  • Varian Unity 600 MHz

Related Database Links:

PDB
DBJ BAG58195 BAG62073 BAJ17684
EMBL CAA27060
GB AAA75493 AAI56756 AAI67147 AAO18082 AAX40997
PRF 1202345A
REF NP_001005862 NP_001276865 NP_001276866 NP_004439 XP_001090319
SP P04626
AlphaFold P04626

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks