BMRB Entry 15211

Name: Solution NMR structure of protein , Northeastprotein yxeF Structural Genomics Consortium target Sr500a
Published: 2007-06-05

Click here to enlarge.

PDB ID: 2joz
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15211
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Solution NMR structure of protein , Northeastprotein yxeF Structural Genomics Consortium target Sr500a

Deposition date:

2007-04-10

Original release date:

2007-06-05

Authors:

Wu, Yibing; Liu, Gaohua; Zhang, Qi; Bhatnagar, Sagar; Chen, Chen X.; Nwosu, Chioma; Xiao, Rong; Cunningham, Kellie; Locke, Jessica; Ma, Li-Chung; Swapna, G.V.T; Baran, Michael C.; Acton, Thomas; Montelione, Gaetano; Szyperski, Thomas

Citation:

Citation: Wu, Yibing; Liu, Gaohua; Zhang, Qi; Bhatnagar, Sagar; Chen, Chen X.; Nwosu, Chioma; Xiao, Rong; Cunningham, Kellie; Locke, Jessica; Ma, Li-Chung; Swapna, G.V.T; Baran, Michael C.; Acton, Thomas; Montelione, Gaetano; Szyperski, Thomas. "Solution NMR structure of protein, Northeastprotein yxeF Structural Genomics Consortium target Sr500a" Protein Sci. ., .-..

Assembly members:

Assembly members:
protein yxeF, polymer, 135 residues, 15450.332 Da.

Natural source:

Natural source: Common Name: not available Taxonomy ID: 1423 Superkingdom: Bacteria Kingdom: Bacillus Genus/species: subtilis subtilis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
protein yxeF: MIMVSGCQQQKEETPFYYGT WDEGRAPGPTDGVKSATVTF TEDEVVETEVMEGRGEVQLP FMAYKVISQSTDGSIEIQYL GPYYPLKSTLKRGENGTLIW EQNGQRKTMTRIESKTGREE KDEKSKSLEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	542
15N chemical shifts	131
1H chemical shifts	886

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	protein yxeF	1

Entities:

Entity 1, protein yxeF 135 residues - 15450.332 Da.

1

MET

ILE

MET

VAL

SER

GLY

CYS

GLN

2

LYS

GLU

THR

PRO

PHE

TYR

GLY

THR

3

TRP

ASP

GLU

GLY

ARG

ALA

PRO

GLY

PRO

THR

4

ASP

GLY

VAL

LYS

SER

ALA

THR

VAL

THR

PHE

5

THR

GLU

ASP

GLU

VAL

GLU

THR

GLU

VAL

6

MET

GLU

GLY

ARG

GLY

GLU

VAL

GLN

LEU

PRO

7

PHE

MET

ALA

TYR

LYS

VAL

ILE

SER

GLN

SER

8

THR

ASP

GLY

SER

ILE

GLU

ILE

GLN

TYR

LEU

9

GLY

PRO

TYR

PRO

LEU

LYS

SER

THR

LEU

10

LYS

ARG

GLY

GLU

ASN

GLY

THR

LEU

ILE

TRP

11

GLU

GLN

ASN

GLY

GLN

ARG

LYS

THR

MET

THR

12

ARG

ILE

GLU

SER

LYS

THR

GLY

ARG

GLU

13

LYS

ASP

GLU

LYS

SER

LYS

SER

LEU

GLU

HIS

14

HIS

Samples:

sample_1: entity, [U-100% 13C; U-100% 15N], 1 mM; Tris 10 mM; NaCl 100%; NaN3 0.02%

sample_2: entity, [U-10% 13C; U-100% 15N], 1 mM; Tris 10 mM; NaCl 100%; NaN3 0.02%

sample_conditions_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
4,3D, GFT HNNCABCA	sample_1	isotropic	sample_conditions_1
4,3D, GFT CABCACONNH	sample_1	isotropic	sample_conditions_1
4,3D, GFT HCCH COSY	sample_1	isotropic	sample_conditions_1
3D, 15N-13C RESOLVED SIMULTANIOUS NOESY	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
1H-13C HSQC	sample_2	isotropic	sample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMRPipe v9.0, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

AutoStruct v1.0, Huang, Swapana, Rajan, Ke, Xia, Shukla, Inouye and Montelione - structure solution

CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

XEASY, Bartels et al. - data analysis, peak picking

NMR spectrometers:

Varian INOVA 750 MHz
Varian Avance 600 MHz

PDB	2JOZ
DBJ	BAA08322 BAI87653 BAM56043 BAM60055 GAK81370
EMBL	CAB15993 CEI59768 CEJ79625 CJR72173 CON90429
GB	ADV94782 AEP93027 AFQ59810 AGA22603 AGG63371
REF	NP_391836 WP_003227095 WP_003244165 WP_014478470 WP_014481402
SP	P54945
AlphaFold	P54945