BMRB Entry 15207

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15207

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Title:
1H, 13C and 15N resonance assignments of YajG
Deposition date:
2007-04-06
Original release date:
2007-08-23
Authors:
Boudet, Julien; Simorre, Jean-Pierre; Bougault, Catherine
Citation:

Citation: Boudet, Julien; Chouquet, Anne; Chahboune, Aicha; Giustini, Cecile; Joris, Bernard; Simorre, Jean-Pierre; Bougault, Catherine. "1H, 13C and 15N resonance assignments of YajG, an Escherichia coli protein of unknown structure and function"  Biomol. NMR Assignments 1, 89-91 (2007).
PubMed: 19636835

Assembly members:

Assembly members:
yajg, polymer, 182 residues, 19990 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: Escherichia   Genus/species: coli coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET26b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
yajg: AKPPTTIEVSPTITLPQQDP SLMGVTVSINGADQRTDQAL AKVTRDNQIVTLTASRDLRF LLQEVLEKQMTARGYMVGPN GPVNLQIIVSQLYADVSQGN VRYNIATKADIAIIATAQNG NKMTKNYRASYNVEGAFQAS NKNIADAVNSVLTDTIADMS QDTSIHEFIKQNARLEHHHH HH

Data sets:
Data typeCount
13C chemical shifts750
15N chemical shifts184
1H chemical shifts1167

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1yajg1

Entities:

Entity 1, yajg 182 residues - 19990 Da.

Residues 175-182 represent a non-native C-terminal histidine tag Signal sequence at the N-terminus of yajg from E. coli (first 18 residues of Swiss-Prot entry P0ADA6) have been deleted.

1   ALALYSPROPROTHRTHRILEGLUVALSER
2   PROTHRILETHRLEUPROGLNGLNASPPRO
3   SERLEUMETGLYVALTHRVALSERILEASN
4   GLYALAASPGLNARGTHRASPGLNALALEU
5   ALALYSVALTHRARGASPASNGLNILEVAL
6   THRLEUTHRALASERARGASPLEUARGPHE
7   LEULEUGLNGLUVALLEUGLULYSGLNMET
8   THRALAARGGLYTYRMETVALGLYPROASN
9   GLYPROVALASNLEUGLNILEILEVALSER
10   GLNLEUTYRALAASPVALSERGLNGLYASN
11   VALARGTYRASNILEALATHRLYSALAASP
12   ILEALAILEILEALATHRALAGLNASNGLY
13   ASNLYSMETTHRLYSASNTYRARGALASER
14   TYRASNVALGLUGLYALAPHEGLNALASER
15   ASNLYSASNILEALAASPALAVALASNSER
16   VALLEUTHRASPTHRILEALAASPMETSER
17   GLNASPTHRSERILEHISGLUPHEILELYS
18   GLNASNALAARGLEUGLUHISHISHISHIS
19   HISHIS

Samples:

sample_1: yajg, [U-100% 13C; U-100% 15N; 80% 2H], 0.85 ± 0.1 mM; sodium phosphate 50 mM; sodium chloride 100 mM; sodium azide 0.02%; H2O 95%; D2O 5%

sample_2: yajg, [U-98% 13C; U-98% 15N], 1 ± 0.2 mM; sodium phosphate 50 mM; sodium chloride 100 mM; sodium azide 0.02%; H2O 90%; D2O 10%

sample_3: yajg, [U-98% 13C; U-98% 15N], 0.8 ± 0.2 mM; sodium phosphate 50 mM; sodium chloride 100 mM; sodium azide 0.02%; D2O 100%

sample_4: yajg, [U-100% 13C; U-100% 15N; 80% 2H], 0.75 ± 0.1 mM; MES 50 mM; sodium chloride 100 mM; sodium azide 0.02%; D2O 5%; H2O 95%

sample_conditions_1: ionic strength: 175 mM; pH: 7.0; pressure: 1 atm; temperature: 310 K

sample_conditions_2: ionic strength: 150 mM; pH: 6.0; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D HBHA(CO)NHsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
3D C(CO)NHsample_2isotropicsample_conditions_1
3D H(CCO)NHsample_2isotropicsample_conditions_1
3D H(C)CH-TOCSYsample_2isotropicsample_conditions_1
3D (H)CCH-TOCSYsample_2isotropicsample_conditions_1
2D Methyl-selective HSQCsample_2isotropicsample_conditions_1
3D Methyl-selective-NOESY-HSQCsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
3D HNCACBsample_4isotropicsample_conditions_2
2D 1H-15N HSQCsample_4isotropicsample_conditions_2
2D 1H-13C HSQCsample_3isotropicsample_conditions_1
3D 1H-13C NOESYsample_3isotropicsample_conditions_1
HBCBCGCDHDsample_3isotropicsample_conditions_1
HBCBCGCDCEHEsample_3isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1

Software:

NMRPipe v2.3, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView v5.2.2, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

VNMRJ v2.1, Varian - collection

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

DBJ BAB33911 BAE76214 BAG75983 BAI23808 BAI29279
EMBL CAP74968 CAQ30906 CAQ97309 CAR01777 CAR06667
GB AAB28883 AAB40190 AAC73537 AAG54784 AAN42034
REF NP_308515 NP_414968 NP_706327 WP_000473497 WP_000473498
SP P0ADA5 P0ADA6
AlphaFold P0ADA5 P0ADA6

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks