BMRB Entry 15204

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15204

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Title:
Backbone assignment of human IgG1 CH3 domain
Deposition date:
2007-04-05
Original release date:
2007-08-23
Authors:
Liu, Dingjiang; Cocco, Melanie; Remmele, Richard; Brems, David
Citation:

Citation: Liu, Dingjiang; Cocco, Melanie; Matsumura, Masazumi; Ren, Da; Becker, Bridget; Remmele, Richard; Brems, David. "Assignment of 1H, 13C and 15N resonances of the reduced human IgG1 C(H)3 domain"  Biomol. NMR Assignments 1, 93-94 (2007).
PubMed: 19636836

Assembly members:

Assembly members:
human_IgG1_CH3_domain, polymer, 107 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
human_IgG1_CH3_domain: GQPREPQVYTLPPSRDELTK NQVSLTCLVKGFYPSDIAVE WESNGQPENNYKTTPPVLDS DGSFFLYSKLTVDKSRWQQG NVFSCSVMHEALHNHYTQKS LSLSPGK

Data sets:
Data typeCount
13C chemical shifts394
15N chemical shifts110
1H chemical shifts610

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CH3 dimer chain 11
2CH3 dimer chain 21

Entities:

Entity 1, CH3 dimer chain 1 107 residues - Formula weight is not available

1   GLYGLNPROARGGLUPROGLNVALTYRTHR
2   LEUPROPROSERARGASPGLULEUTHRLYS
3   ASNGLNVALSERLEUTHRCYSLEUVALLYS
4   GLYPHETYRPROSERASPILEALAVALGLU
5   TRPGLUSERASNGLYGLNPROGLUASNASN
6   TYRLYSTHRTHRPROPROVALLEUASPSER
7   ASPGLYSERPHEPHELEUTYRSERLYSLEU
8   THRVALASPLYSSERARGTRPGLNGLNGLY
9   ASNVALPHESERCYSSERVALMETHISGLU
10   ALALEUHISASNHISTYRTHRGLNLYSSER
11   LEUSERLEUSERPROGLYLYS

Samples:

sample_1: human IgG1 CH3 domain, [U-100% 13C; U-100% 15N], 0.5 mM; sodium acetate 10 mM

sample_conditions_1: ionic strength: 0.02 M; pH: 5.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - data analysis

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
EMBL CAA43606 CAM91874
GB AAD38158 AAF42993 AAG00448 ABD72617 ABQ01159
PIR A36040 S69339 S69340
PRF 1605217A

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks