BMRB Entry 15201
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15201
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NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Tafer, Hakim; Hiller, Sebastian; Hilty, Christian; Fernandez, Cesar; Wuthrich, Kurt. "Nonrandom Structure in the Urea-Unfolded Escherichia coli Outer Membrane Protein X (OmpX)" Biochemistry 43, 860-869 (2004).
PubMed: 14744128
Assembly members:
Outer_membrane_protein_X, polymer, 148 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21
Entity Sequences (FASTA):
Outer_membrane_protein_X: ATSTVTGGYAQSDAQGQMNK
MGGFNLKYRYEEDNSPLGVI
GSFTYTEKSRTASSGDYNKN
QYYGITAGPAYRINDWASIY
GVVGVGYGKFQTTEYPTYKH
DTSDYGFSYGAGLQFNPMEN
VALDFSYEQSRIRSVDVGTW
IAGVGYRF
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 550 |
| 15N chemical shifts | 146 |
| 1H chemical shifts | 869 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | OmpX | 1 |
Entities:
Entity 1, OmpX 148 residues - Formula weight is not available
| 1 | ALA | THR | SER | THR | VAL | THR | GLY | GLY | TYR | ALA | ||||
| 2 | GLN | SER | ASP | ALA | GLN | GLY | GLN | MET | ASN | LYS | ||||
| 3 | MET | GLY | GLY | PHE | ASN | LEU | LYS | TYR | ARG | TYR | ||||
| 4 | GLU | GLU | ASP | ASN | SER | PRO | LEU | GLY | VAL | ILE | ||||
| 5 | GLY | SER | PHE | THR | TYR | THR | GLU | LYS | SER | ARG | ||||
| 6 | THR | ALA | SER | SER | GLY | ASP | TYR | ASN | LYS | ASN | ||||
| 7 | GLN | TYR | TYR | GLY | ILE | THR | ALA | GLY | PRO | ALA | ||||
| 8 | TYR | ARG | ILE | ASN | ASP | TRP | ALA | SER | ILE | TYR | ||||
| 9 | GLY | VAL | VAL | GLY | VAL | GLY | TYR | GLY | LYS | PHE | ||||
| 10 | GLN | THR | THR | GLU | TYR | PRO | THR | TYR | LYS | HIS | ||||
| 11 | ASP | THR | SER | ASP | TYR | GLY | PHE | SER | TYR | GLY | ||||
| 12 | ALA | GLY | LEU | GLN | PHE | ASN | PRO | MET | GLU | ASN | ||||
| 13 | VAL | ALA | LEU | ASP | PHE | SER | TYR | GLU | GLN | SER | ||||
| 14 | ARG | ILE | ARG | SER | VAL | ASP | VAL | GLY | THR | TRP | ||||
| 15 | ILE | ALA | GLY | VAL | GLY | TYR | ARG | PHE |
Samples:
sample_1: Outer membrane protein X, [U-100% 13C; U-100% 15N], 3 mM; urea 8 M; D2O, [U-100% 2H], 5%; sodium phosphate 20 mM; sodium azide 0.1 mM
sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 288 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
XEASY, Bartels et al. - chemical shift assignment
NMR spectrometers:
- Bruker DRX 750 MHz
Related Database Links:
| BMRB | 18796 18797 19892 |
| PDB | |
| DBJ | BAA35486 BAB34315 BAG76394 BAI24257 BAI29701 |
| EMBL | CAP75284 CAQ31315 CAQ97717 CAR02170 CAR06985 |
| GB | AAA21856 AAA66329 AAC73901 AAG55186 AAN42399 |
| REF | NP_308919 NP_415335 NP_706692 WP_001295296 WP_001340109 |
| SP | P0A917 P0A918 P0A919 P0A920 |
| AlphaFold | P0A917 P0A918 P0A919 P0A920 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks