BMRB Entry 15194

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15194

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Title:
1H, 13C and 15N resonance assignment of truncated SUMO from Trypanosoma brucei
Deposition date:
2007-03-21
Original release date:
2007-08-23
Authors:
Shang, Qiang; Tu, Xiaoming
Citation:

Citation: Shang, Qiang; Lin, Lin; Zhang, Jiahai; Tu, Xiaoming. "1H, 13C and 15N resonance assignment of truncated SUMO from Trypanosoma brucei"  Biomol. NMR Assignments 1, 103-104 (2007).
PubMed: 19636839

Assembly members:

Assembly members:
small_ubiquitin-like_modifier, polymer, 110 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Trypanosoma brucei   Taxonomy ID: 5691   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Trypanosoma brucei

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
small_ubiquitin-like_modifier: MSNNGGEPSNNGGEGAEGTC KEETALVAVKVVNADGAEMF FRIKSRTALKKLIDTYCKKQ GISRNSVRFLFDGTPIDETK TPEELGMEDDDVIDAMVEQT GGLGHHHHHH

Data sets:
Data typeCount
13C chemical shifts375
15N chemical shifts99
1H chemical shifts562

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1small ubiquitin-like modifier1

Entities:

Entity 1, small ubiquitin-like modifier 110 residues - Formula weight is not available

1   METSERASNASNGLYGLYGLUPROSERASN
2   ASNGLYGLYGLUGLYALAGLUGLYTHRCYS
3   LYSGLUGLUTHRALALEUVALALAVALLYS
4   VALVALASNALAASPGLYALAGLUMETPHE
5   PHEARGILELYSSERARGTHRALALEULYS
6   LYSLEUILEASPTHRTYRCYSLYSLYSGLN
7   GLYILESERARGASNSERVALARGPHELEU
8   PHEASPGLYTHRPROILEASPGLUTHRLYS
9   THRPROGLUGLULEUGLYMETGLUASPASP
10   ASPVALILEASPALAMETVALGLUGLNTHR
11   GLYGLYLEUGLYHISHISHISHISHISHIS

Samples:

sample_1: small ubiquitin-like modifier, [U-100% 13C; U-100% 15N], 0.8 mM; EDTA 1.5 mM; D2O, [U-100% 2H], 10%; PMSF 1 mM; sodium chloride 100 mM; phosphate 50 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.25; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker DMX 500 MHz

Related Database Links:

PDB
EMBL CBH11308
GB AAX79561 AAZ11434
REF XP_011773595 XP_844993

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks