BMRB Entry 15159

Name: Mouse Itch 3rd domain phosphorilated in T30
Published: 2007-10-24

Click here to enlarge.

PDB ID: 2joc
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR15159
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Mouse Itch 3rd domain phosphorilated in T30

Deposition date:

2007-03-06

Original release date:

2007-10-24

Authors:

Macias, Maria; Shaw, Alison; Martin-Malpartida, Pau; Morales, Begonya; Ruiz, Lidia; Ramirez-Espain, Ximena; Yraola, Francesc; Royo, Miriam

Citation:

Citation: Morales, Begonya; Ramirez-Espain, Ximena; Shaw, Alison; Martin-Malpartida, Pau; Yraola, Francesc; Sanchez-Tillo, Ester; Farrera, Consol; Celada, Antonio; Royo, Miriam; Macias, Maria. "NMR structural studies of the ItchWW3 domain reveal that phosphorylation at T30 inhibits the interaction with PPxY-containing ligands." Structure 15, 473-483 (2007).
PubMed: 17437719

Assembly members:

Assembly members:
PhosphoI3, polymer, 37 residues, 4360.759 Da.

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PhosphoI3: GAMGPLPPGWEKRTDSNGRV YFVNHNTRIXQWEDPRS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
spectral_peak_list
- peak_list_1
- peak_list_2

Data type	Count
1H chemical shifts	240

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PhosphoI3	1

Entities:

Entity 1, PhosphoI3 37 residues - 4360.759 Da.

1

GLY

ALA

MET

GLY

PRO

LEU

PRO

GLY

TRP

2

GLU

LYS

ARG

THR

ASP

SER

ASN

GLY

ARG

VAL

3

TYR

PHE

VAL

ASN

HIS

ASN

THR

ARG

ILE

TPO

4

GLN

TRP

GLU

ASP

PRO

ARG

SER

Samples:

sample_1: PhosphoI3 1.0 mM; Sodium Phosphate 20 mM; NaCl 100 mM; Sodium Azide 0.02 % v/v

sample_conditions_1: ionic strength: 0.4 M; pH: 6.5; pressure: 1 atm; temperature: 280 K

sample_conditions_2: ionic strength: 0.4 M; pH: 6.5; pressure: 1 atm; temperature: 285 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_2
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_2

Software:

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

ARIA, Linge, O'Donoghue and Nilges - structure solution

NMR spectrometers:

Bruker DRX 600 MHz