BMRB Entry 15142

Name: Solution structure of dynein light chain 2A
Published: 2009-10-15

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PDB ID: 2e8j
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15142
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of dynein light chain 2A

Deposition date:

2007-02-24

Original release date:

2009-10-15

Authors:

Kouno, T.; Nabeshima, Y.; Mizuguchi, M.; Kawano, K.

Citation:

Citation: Kouno, T.; Nabeshima, Y.; Mizuguchi, M.; Kawano, K.. "Solution structure of dynein light chain 2A" .

Assembly members:

Assembly members:
Dynein_light_chain_2A,_cytoplasmic, polymer, 101 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-6P

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Dynein_light_chain_2A,_cytoplasmic: GPLGSMAEVEETLKRLQSQK GVQGIIVVNTEGIPIKSTMD NPTTTQYASLMHSFILKARS TVRDIDPQNDLTFLRIRSKK NEIMVAPDKDYFLIVIQNPT E

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	305
15N chemical shifts	90
1H chemical shifts	649

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	chain A	1
2	chain B	1

Entities:

Entity 1, chain A 101 residues - Formula weight is not available

1

GLY

PRO

LEU

GLY

SER

MET

ALA

GLU

VAL

GLU

2

GLU

THR

LEU

LYS

ARG

LEU

GLN

SER

GLN

LYS

3

GLY

VAL

GLN

GLY

ILE

VAL

ASN

THR

4

GLU

GLY

ILE

PRO

ILE

LYS

SER

THR

MET

ASP

5

ASN

PRO

THR

GLN

TYR

ALA

SER

LEU

6

MET

HIS

SER

PHE

ILE

LEU

LYS

ALA

ARG

SER

7

THR

VAL

ARG

ASP

ILE

ASP

PRO

GLN

ASN

ASP

8

LEU

THR

PHE

LEU

ARG

ILE

ARG

SER

LYS

9

ASN

GLU

ILE

MET

VAL

ALA

PRO

ASP

LYS

ASP

10

TYR

PHE

LEU

ILE

VAL

ILE

GLN

ASN

PRO

THR

11

GLU

Samples:

sample_1: Dynein light chain 2A, cytoplasmic, [U-100% 13C; U-100% 15N], 0.6 mM; phosphate buffer 20 mM; NaCl 0.1 mM; EDTA 20 mM; H2O 95%; D2O 5%

sample_2: Dynein light chain 2A, cytoplasmic, [U-100% 13C; U-100% 15N], 0.6 mM; phosphate buffer 20 mM; NaCl 0.1 mM; EDTA 20 mM; D2O 100%

sample_conditions_1: ionic strength: 40 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D_15N-separated_NOESY	sample_1	isotropic	sample_conditions_1
3D_13C-separated_NOESY	sample_2	isotropic	sample_conditions_1
2D NOESY	sample_2	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-COSY	sample_2	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1

Software:

NMRPipe v2.2, Delaglio - data analysis

PIPP v4.3.2, Garrett - data analysis

X-PLOR v3.1f, Brunger - refinement

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	1Z09 2B95 2E8J 2HZ5
DBJ	BAG57523
GB	AAF29126 AAF86646 AAH02481 AAK18712 AAK95342
REF	NP_001177109 NP_001268656 NP_001268657 NP_001268658 NP_054902
SP	Q9NP97
AlphaFold	Q9NP97