BMRB Entry 15141
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15141
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Gall, Chris; Xu, Hanyu; Brickenden, Anne; Ai, Xuanjun; Choy, Wing-Yiu. "The intrinsically disordered TC-1 interacts with Chibby via regions with high helical propensity" Biochemistry 16, 2510-2518 (2007).
PubMed: 17905836
Assembly members:
Thyroid_Cancer_1_(TC-1)_protein, polymer, 106 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b
Entity Sequences (FASTA):
Thyroid_Cancer_1_(TC-1)_protein: MKAKRSHQAIIMSTSLRVSP
SIHGYHFDTASRKKAVGNIF
ENTDQESLERLFRNSGDKKA
EERAKIIFAIDQDVEEKTRA
LMALKKRTKDKLFQFLKLRK
YSIKVH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 233 |
| 15N chemical shifts | 75 |
| 1H chemical shifts | 75 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TC-1 | 1 |
Entities:
Entity 1, TC-1 106 residues - Formula weight is not available
| 1 | MET | LYS | ALA | LYS | ARG | SER | HIS | GLN | ALA | ILE | ||||
| 2 | ILE | MET | SER | THR | SER | LEU | ARG | VAL | SER | PRO | ||||
| 3 | SER | ILE | HIS | GLY | TYR | HIS | PHE | ASP | THR | ALA | ||||
| 4 | SER | ARG | LYS | LYS | ALA | VAL | GLY | ASN | ILE | PHE | ||||
| 5 | GLU | ASN | THR | ASP | GLN | GLU | SER | LEU | GLU | ARG | ||||
| 6 | LEU | PHE | ARG | ASN | SER | GLY | ASP | LYS | LYS | ALA | ||||
| 7 | GLU | GLU | ARG | ALA | LYS | ILE | ILE | PHE | ALA | ILE | ||||
| 8 | ASP | GLN | ASP | VAL | GLU | GLU | LYS | THR | ARG | ALA | ||||
| 9 | LEU | MET | ALA | LEU | LYS | LYS | ARG | THR | LYS | ASP | ||||
| 10 | LYS | LEU | PHE | GLN | PHE | LEU | LYS | LEU | ARG | LYS | ||||
| 11 | TYR | SER | ILE | LYS | VAL | HIS |
Samples:
Thyroid_Cancer_1: Thyroid Cancer 1 (TC-1) protein, [U-99% 13C; U-99% 15N], 0.1 mM; DSS5 – 10 mM; sodium acetate 10 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 10 mM; pH: 5.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCACB | Thyroid_Cancer_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | Thyroid_Cancer_1 | isotropic | sample_conditions_1 |
| 3D HNCO | Thyroid_Cancer_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | Thyroid_Cancer_1 | isotropic | sample_conditions_1 |
Software:
NMRView, Johnson, One Moon Scientific - chemical shift assignment
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| GB | AAF78961 AAH20623 AAH21672 ACE87044 ACE87727 |
| REF | NP_001180920 NP_064515 XP_002819077 XP_003734646 XP_003823354 |
| SP | Q9NR00 |
| AlphaFold | Q9NR00 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks