BMRB Entry 15127

Name: Chemical shift and constraint files for the RegB ribonuclease
Published: 2007-07-16

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR15127

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical shift and constraint files for the RegB ribonuclease

Deposition date:

2007-02-02

Original release date:

2007-07-16

Authors:

Odaert, Benoit; Saida, Fakhri; Uzan, Marc; Bontems, Francois

Citation:

Citation: Odaert, Benoit; Saida, Fakhri; Aliprandi, Pascale; Durand, Sylvain; Crechet, Jean-Bernard; Guerois, Raphael; Laalami, Soumaya; Uzan, Marc; Bontems, Francois. "Structural and functional studies of RegB, a new member of a family of sequence-specific ribonucleases involved in mRNA inactivation on the ribosome." J. Biol. Chem. 282, 2019-2028 (2007).
PubMed: 17046813

Assembly members:

Assembly members:
RegB, polymer, 153 residues, Formula weight is not available

Natural source:

Natural source: Common Name: bacteriophage T4 Taxonomy ID: 38018 Superkingdom: Viruses Kingdom: not available Genus/species: not available Bacteriophage

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pTOXR-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RegB: MTINTEVFIRRNKLRRHFES EFRQINNEIREASKAAGVSS FHLKYSQALLDRAIQREIDE TYVFELFHKIKDHVLEVNEF LSMPPRPDIDEDFIDGVEYR PGRLEITDGNLWLGFTVCKP NEKFKDPSLQCRMAIINSRR LPGKASKAVIKTQ

Data sets:

assigned_chemical_shifts

Data type	Count
13C chemical shifts	488
15N chemical shifts	320
1H chemical shifts	1959

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RegB	1

Entities:

Entity 1, RegB 153 residues - Formula weight is not available

1

MET

THR

ILE

ASN

THR

GLU

VAL

PHE

ILE

ARG

2

ARG

ASN

LYS

LEU

ARG

HIS

PHE

GLU

SER

3

GLU

PHE

ARG

GLN

ILE

ASN

GLU

ILE

ARG

4

GLU

ALA

SER

LYS

ALA

GLY

VAL

SER

5

PHE

HIS

LEU

LYS

TYR

SER

GLN

ALA

LEU

6

ASP

ARG

ALA

ILE

GLN

ARG

GLU

ILE

ASP

GLU

7

THR

TYR

VAL

PHE

GLU

LEU

PHE

HIS

LYS

ILE

8

LYS

ASP

HIS

VAL

LEU

GLU

VAL

ASN

GLU

PHE

9

LEU

SER

MET

PRO

ARG

PRO

ASP

ILE

ASP

10

GLU

ASP

PHE

ILE

ASP

GLY

VAL

GLU

TYR

ARG

11

PRO

GLY

ARG

LEU

GLU

ILE

THR

ASP

GLY

ASN

12

LEU

TRP

LEU

GLY

PHE

THR

VAL

CYS

LYS

PRO

13

ASN

GLU

LYS

PHE

LYS

ASP

PRO

SER

LEU

GLN

14

CYS

ARG

MET

ALA

ILE

ASN

SER

ARG

15

LEU

PRO

GLY

LYS

ALA

SER

LYS

ALA

VAL

ILE

16

LYS

THR

GLN

Samples:

RegB_15N_H2O: RegB, [U-15N], 600 – 800 uM; Citrate 50 mM; NaCl 300 mM; DTT 2.0 mM; H2O 95%; D2O 5%

RegB_15N_D2O: RegB, [U-15N], 600 – 800 uM; Citrate 50 mM; NaCl 300 mM; DTT 2.0 mM; D2O 100%

RegB_13C15N_H2O: RegB, [U-100% 13C; U-100% 15N], 600 – 800 uM; Citrate 50 mM; NaCl 300 mM; DTT 2.0 mM; H2O 95%; D2O 5%

RegB_13C15N_D2O: RegB, [U-100% 13C; U-100% 15N], 600 – 800 uM; Citrate 50 mM; NaCl 300 mM; DTT 2.0 mM; D2O 100%

Citrate_pH6.0: pH: 6.0; pressure: 1 atm; temperature: 305 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	RegB_15N_H2O	isotropic	Citrate_pH6.0
2D 1H-13C HSQC	RegB_13C15N_D2O	isotropic	Citrate_pH6.0
3D HNCO	RegB_13C15N_H2O	isotropic	Citrate_pH6.0
3D HNCA	RegB_13C15N_H2O	isotropic	Citrate_pH6.0
3D HNCACB	RegB_13C15N_H2O	isotropic	Citrate_pH6.0
3D HN(CO)CA	RegB_13C15N_H2O	isotropic	Citrate_pH6.0
3D CBCA(CO)NH	RegB_13C15N_H2O	isotropic	Citrate_pH6.0
3D HBHA(CO)NH	RegB_13C15N_H2O	isotropic	Citrate_pH6.0
3D HCCH-TOCSY	RegB_13C15N_D2O	isotropic	Citrate_pH6.0
3D 15N-NOESY-HSQC	RegB_15N_H2O	isotropic	Citrate_pH6.0
3D 13C-NOESY-HSQC	RegB_13C15N_H2O	isotropic	Citrate_pH6.0
3D 13C-NOESY-HSQC	RegB_13C15N_D2O	isotropic	Citrate_pH6.0
2D NOESY	RegB_15N_D2O	isotropic	Citrate_pH6.0
2D TOCSY	RegB_15N_D2O	isotropic	Citrate_pH6.0
2D 1H-15N HSQC	RegB_13C15N_H2O	isotropic	Citrate_pH6.0
3D 13Caro-NOESY-HSQC	RegB_13C15N_D2O	isotropic	Citrate_pH6.0

Software:

xwinnmr v3.0, Bruker Biospin - Spectra processing

SPARKY v3.113, Thomas L. Goddard - Data analysis

INCA, Bernard Gilquin - Structure Calculation

NMR spectrometers:

Bruker DRX 600 MHz
Bruker DRX 800 MHz

GB	AAD42652 AAD42652 ABI94940 ACP30763 ACP31038 ADJ39833
REF	NP_049726 NP_049726 YP_002854075 YP_002854455 YP_004415013 YP_006986670
PDB	2HX6
DBJ	BAI83127
EMBL	CAA28227 CAD32702 CAD32704 CAD32706 CAD32708
SP	P13312
AlphaFold	P13312