BMRB Entry 15123

Title:
SeV Ntail(443-501)
Deposition date:
2007-02-01
Original release date:
2007-05-14
Authors:
Houben, Klaartje; Marion, Dominique; Tarbouriech, Nicolas; Ruigrok, Rob; Blanchard, Laurence
Citation:

Citation: Houben, Klaartje; Marion, Dominique; Tarbouriech, Nicolas; Ruigrok, Rob; Blanchard, Laurence. "Interaction of the C-terminal domains of sendai virus N and P proteins: comparison of polymerase-nucleocapsid interactions within the paramyxovirus family"  J. Virol. 81, 6807-6816 (2007).
PubMed: 17459940

Assembly members:

Assembly members:
SeV_nucleoprotein_Ntail, polymer, 59 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Respirovirus   Taxonomy ID: 186938   Superkingdom: Viruses   Kingdom: not available   Genus/species: Respirovirus not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-TEV

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SeV_nucleoprotein_Ntail: GESGERWARQVSGGHFVTLH GAERLEEETNDEDVSDIERR IAMRLAERRQEDSATHGDE

Data sets:
Data typeCount
13C chemical shifts162
15N chemical shifts70
1H chemical shifts357

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ntail1

Entities:

Entity 1, Ntail 59 residues - Formula weight is not available

1   GLYGLUSERGLYGLUARGTRPALAARGGLN
2   VALSERGLYGLYHISPHEVALTHRLEUHIS
3   GLYALAGLUARGLEUGLUGLUGLUTHRASN
4   ASPGLUASPVALSERASPILEGLUARGARG
5   ILEALAMETARGLEUALAGLUARGARGGLN
6   GLUASPSERALATHRHISGLYASPGLU

Samples:

13C_15N: SeV nucleoprotein Ntail, [U-100% 13C; U-100% 15N], 1 mM; sodium chloride 500 mM; potassium phosphate 50 mM

sample_condition_1: ionic strength: 0.5 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC13C_15Nisotropicsample_condition_1
3D CBCA(CO)NH13C_15Nisotropicsample_condition_1
3D HCCH-TOCSY13C_15Nisotropicsample_condition_1
3D C(CO)NH13C_15Nisotropicsample_condition_1
3D HNCACB13C_15Nisotropicsample_condition_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Varian Avance 600 MHz
  • Varian Avance 800 MHz

Related Database Links:

DBJ BAD74219 BAD74225 BAF73479 BAF73485 BAH59027
EMBL CAA24945 CAA35092 CAA35715
GB AAB06278 AAB06284 AAB06290
SP P04857 P04858 Q07097
AlphaFold P04857 P04858 Q07097

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks