BMRB Entry 15121

Name: Backbone 1H, 13C and 15N resonance assignments for the 26kD human deubiquitinating enzyme UCH-L3
Published: 2007-06-27

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15121

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C and 15N resonance assignments for the 26kD human deubiquitinating enzyme UCH-L3

Deposition date:

2007-01-31

Original release date:

2007-06-27

Authors:

Harris, Richard; Hommel, Ulrich; Driscoll, Paul

Citation:

Citation: Harris, Richard; Eidhoff, Ulf; Vinzenz, Daniela; Renatus, Martin; Gerhartz, Bernd; Hommel, Ulrich; Driscoll, Paul. "Backbone 1H, 13C, and 15N resonance assignments for the 26-kD human de-ubiquitinating enzyme UCH-L3" Biomol. NMR Assignments 1, 51-53 (2007).
PubMed: 19636824

Assembly members:

Assembly members:
UCH-L3, polymer, 230 residues, 26182 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
UCH-L3: MEGQRWLPLEANPEVTNQFL KQLGLHPNWQFVDVYGMDPE LLSMVPRPVCAVLLLFPITE KYEVFRTEEEEKIKSQGQDV TSSVYFMKQTISNACGTIGL IHAIANNKDKMHFESGSTLK KFLEESVSMSPEERARYLEN YDAIRVTHETSAHEGQTEAP SIDEKVDLHFIALVHVDGHL YELDGRKPFPINHGETSDET LLEDAIEVCKKFMERDPDEL RFNAIALSAA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	591
15N chemical shifts	186
1H chemical shifts	186

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	UCH-L3	1

Entities:

Entity 1, UCH-L3 230 residues - 26182 Da.

1	MET	GLU	GLY	GLN	ARG	TRP	LEU	PRO	LEU	GLU
2	ALA	ASN	PRO	GLU	VAL	THR	ASN	GLN	PHE	LEU
3	LYS	GLN	LEU	GLY	LEU	HIS	PRO	ASN	TRP	GLN
4	PHE	VAL	ASP	VAL	TYR	GLY	MET	ASP	PRO	GLU
5	LEU	LEU	SER	MET	VAL	PRO	ARG	PRO	VAL	CYS
6	ALA	VAL	LEU	LEU	LEU	PHE	PRO	ILE	THR	GLU
7	LYS	TYR	GLU	VAL	PHE	ARG	THR	GLU	GLU	GLU
8	GLU	LYS	ILE	LYS	SER	GLN	GLY	GLN	ASP	VAL
9	THR	SER	SER	VAL	TYR	PHE	MET	LYS	GLN	THR
10	ILE	SER	ASN	ALA	CYS	GLY	THR	ILE	GLY	LEU
11	ILE	HIS	ALA	ILE	ALA	ASN	ASN	LYS	ASP	LYS
12	MET	HIS	PHE	GLU	SER	GLY	SER	THR	LEU	LYS
13	LYS	PHE	LEU	GLU	GLU	SER	VAL	SER	MET	SER
14	PRO	GLU	GLU	ARG	ALA	ARG	TYR	LEU	GLU	ASN
15	TYR	ASP	ALA	ILE	ARG	VAL	THR	HIS	GLU	THR
16	SER	ALA	HIS	GLU	GLY	GLN	THR	GLU	ALA	PRO
17	SER	ILE	ASP	GLU	LYS	VAL	ASP	LEU	HIS	PHE
18	ILE	ALA	LEU	VAL	HIS	VAL	ASP	GLY	HIS	LEU
19	TYR	GLU	LEU	ASP	GLY	ARG	LYS	PRO	PHE	PRO
20	ILE	ASN	HIS	GLY	GLU	THR	SER	ASP	GLU	THR
21	LEU	LEU	GLU	ASP	ALA	ILE	GLU	VAL	CYS	LYS
22	LYS	PHE	MET	GLU	ARG	ASP	PRO	ASP	GLU	LEU
23	ARG	PHE	ASN	ALA	ILE	ALA	LEU	SER	ALA	ALA

Samples:

sample_1: UCH-L3, [U-100% 13C; U-100% 15N; 80% 2H], 1 mM

sample_conditions_1: ionic strength: 25 mM; pH: 7; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CA)CB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HN(COCA)CB	sample_1	isotropic	sample_conditions_1

Software:

ANSIG, Kraulis - chemical shift assignment

NMR spectrometers:

Varian UnityPlus 500 MHz

PDB	1UCH 1XD3
DBJ	BAB20094 BAB47136 BAC34161 BAG36417
EMBL	CAG33136 CAH93427
GB	AAA36791 AAF64193 AAH18125 AAH48481 AAI10248
REF	NP_001035631 NP_001070695 NP_001103635 NP_001253095 NP_001257881
SP	P15374 Q06AB3 Q2TBG8 Q91Y78 Q9JKB1
TPG	DAA23822
AlphaFold	P15374 Q06AB3 Q2TBG8 Q91Y78 Q9JKB1

1	MET	GLU	GLY	GLN	ARG	TRP	LEU	PRO	LEU	GLU
2	ALA	ASN	PRO	GLU	VAL	THR	ASN	GLN	PHE	LEU
3	LYS	GLN	LEU	GLY	LEU	HIS	PRO	ASN	TRP	GLN
4	PHE	VAL	ASP	VAL	TYR	GLY	MET	ASP	PRO	GLU
5	LEU	LEU	SER	MET	VAL	PRO	ARG	PRO	VAL	CYS
6	ALA	VAL	LEU	LEU	LEU	PHE	PRO	ILE	THR	GLU
7	LYS	TYR	GLU	VAL	PHE	ARG	THR	GLU	GLU	GLU
8	GLU	LYS	ILE	LYS	SER	GLN	GLY	GLN	ASP	VAL
9	THR	SER	SER	VAL	TYR	PHE	MET	LYS	GLN	THR
10	ILE	SER	ASN	ALA	CYS	GLY	THR	ILE	GLY	LEU
11	ILE	HIS	ALA	ILE	ALA	ASN	ASN	LYS	ASP	LYS
12	MET	HIS	PHE	GLU	SER	GLY	SER	THR	LEU	LYS
13	LYS	PHE	LEU	GLU	GLU	SER	VAL	SER	MET	SER
14	PRO	GLU	GLU	ARG	ALA	ARG	TYR	LEU	GLU	ASN
15	TYR	ASP	ALA	ILE	ARG	VAL	THR	HIS	GLU	THR
16	SER	ALA	HIS	GLU	GLY	GLN	THR	GLU	ALA	PRO
17	SER	ILE	ASP	GLU	LYS	VAL	ASP	LEU	HIS	PHE
18	ILE	ALA	LEU	VAL	HIS	VAL	ASP	GLY	HIS	LEU
19	TYR	GLU	LEU	ASP	GLY	ARG	LYS	PRO	PHE	PRO
20	ILE	ASN	HIS	GLY	GLU	THR	SER	ASP	GLU	THR
21	LEU	LEU	GLU	ASP	ALA	ILE	GLU	VAL	CYS	LYS
22	LYS	PHE	MET	GLU	ARG	ASP	PRO	ASP	GLU	LEU
23	ARG	PHE	ASN	ALA	ILE	ALA	LEU	SER	ALA	ALA

1	MET	GLU	GLY	GLN	ARG	TRP	LEU	PRO	LEU	GLU
2	ALA	ASN	PRO	GLU	VAL	THR	ASN	GLN	PHE	LEU
3	LYS	GLN	LEU	GLY	LEU	HIS	PRO	ASN	TRP	GLN
4	PHE	VAL	ASP	VAL	TYR	GLY	MET	ASP	PRO	GLU
5	LEU	LEU	SER	MET	VAL	PRO	ARG	PRO	VAL	CYS
6	ALA	VAL	LEU	LEU	LEU	PHE	PRO	ILE	THR	GLU
7	LYS	TYR	GLU	VAL	PHE	ARG	THR	GLU	GLU	GLU
8	GLU	LYS	ILE	LYS	SER	GLN	GLY	GLN	ASP	VAL
9	THR	SER	SER	VAL	TYR	PHE	MET	LYS	GLN	THR
10	ILE	SER	ASN	ALA	CYS	GLY	THR	ILE	GLY	LEU
11	ILE	HIS	ALA	ILE	ALA	ASN	ASN	LYS	ASP	LYS
12	MET	HIS	PHE	GLU	SER	GLY	SER	THR	LEU	LYS
13	LYS	PHE	LEU	GLU	GLU	SER	VAL	SER	MET	SER
14	PRO	GLU	GLU	ARG	ALA	ARG	TYR	LEU	GLU	ASN
15	TYR	ASP	ALA	ILE	ARG	VAL	THR	HIS	GLU	THR
16	SER	ALA	HIS	GLU	GLY	GLN	THR	GLU	ALA	PRO
17	SER	ILE	ASP	GLU	LYS	VAL	ASP	LEU	HIS	PHE
18	ILE	ALA	LEU	VAL	HIS	VAL	ASP	GLY	HIS	LEU
19	TYR	GLU	LEU	ASP	GLY	ARG	LYS	PRO	PHE	PRO
20	ILE	ASN	HIS	GLY	GLU	THR	SER	ASP	GLU	THR
21	LEU	LEU	GLU	ASP	ALA	ILE	GLU	VAL	CYS	LYS
22	LYS	PHE	MET	GLU	ARG	ASP	PRO	ASP	GLU	LEU
23	ARG	PHE	ASN	ALA	ILE	ALA	LEU	SER	ALA	ALA