BMRB Entry 15117

Title:
Backbone H, C, N Chemical Shifts for Influenza A NS1 (1-73) Protein Bound to dsRNA   PubMed: 17475623
Deposition date:
2007-01-29
Original release date:
2008-02-11
Authors:
Yin, Cuifeng; Montelione, Gaetano
Citation:

Citation: Yin, Cuifeng; Khan, Javed; Swapna, Gurla; Ertekin, Asli; Krug, Robert; Tong, Liang; Montelione, Gaetano. "Conserved surface features form the double-stranded RNA binding site of non-structural protein 1 (NS1) from influenza A and B viruses."  J. Biol. Chem. 282, 20584-20592 (2007).

Assembly members:

Assembly members:
NS1A, polymer, 73 residues, 8297.416 Da.
stranded_RNA_1, polymer, 16 residues, Formula weight is not available
stranded_RNA_2, polymer, 16 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: influenza A virus A/Udorn/72   Taxonomy ID: 11320   Superkingdom: Viruses   Kingdom: not available   Genus/species: Influenzavirus A Influenza A virus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a

Experimental source:

Natural source:   Common Name: influenza A virus A/Udorn/72   Taxonomy ID: 11320   Superkingdom: Viruses   Kingdom: not available   Genus/species: Influenzavirus A Influenza A virus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts235
15N chemical shifts87
1H chemical shifts88

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NS1A_chain 11
2NS1A_chain 21
3dsRNA2
4dsRNA3

Entities:

Entity 1, NS1A_chain 1 73 residues - 8297.416 Da.

1   METASPSERASNTHRVALSERSERPHEGLN
2   VALASPCYSPHELEUTRPHISVALARGLYS
3   GLNVALVALASPGLNGLULEUGLYASPALA
4   PROPHELEUASPARGLEUARGARGASPGLN
5   LYSSERLEUARGGLYARGGLYSERTHRLEU
6   GLYLEUASNILEGLUALAALATHRHISVAL
7   GLYLYSGLNILEVALGLULYSILELEULYS
8   GLUGLUSER

Entity 2, dsRNA 16 residues - Formula weight is not available

1   CCAUCCUCUA
2   CAGGCG

Entity 3, dsRNA 16 residues - Formula weight is not available

1   CCAUCCUCUA
2   CAGGCG

Samples:

NS1A_(1-73): NS1A, [U-100% 13C; U-100% 15N], 0.1 mM; ammonium acetate 50 mM; ds-RNA 0.2 mM

sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCNS1A_(1-73)isotropicsample_conditions_1
3D HNCONS1A_(1-73)isotropicsample_conditions_1
3D HNCANS1A_(1-73)isotropicsample_conditions_1
3D HNCACBNS1A_(1-73)isotropicsample_conditions_1
3D HN(CO)CACBNS1A_(1-73)isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

AutoAssign, Zimmerman, Moseley, Kulikowski, Montelione - data analysis

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Varian INOVA 600 MHz

Related Database Links:

BMRB 4317
PDB
DBJ BAA01428
EMBL CAA24288 CAC09426
GB AAA43086 AAA43515 AAA43688 AAC36136 AAC36138
PRF 2005320B 2005320D
SP P03494 P03495 P69277 P69278 Q1PUD3

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts