BMRB Entry 15114

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15114

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Title:
Solution structure of L8A mutant of HIV-1 myristoylated matrix protein
Deposition date:
2007-01-27
Original release date:
2007-03-06
Authors:
Saad, J.; Loeliger, E.; Luncsford, P.; Liriano, M.; Tai, J.; Kim, A.; Miller, J.; Joshi, A.; Freed, E.; Summers, M.
Citation:

Citation: Saad, J.; Loeliger, E.; Luncsford, P.; Liriano, M.; Tai, J.; Kim, A.; Miller, J.; Joshi, A.; Freed, E.; Summers, M.. "Point Mutations in the HIV-1 Matrix Protein Turn Off the Myristyl Switch."  J. Mol. Biol. 366, 574-585 (2007).
PubMed: 17188710

Assembly members:

Assembly members:
Gag_polyprotein, polymer, 131 residues, Formula weight is not available
MYRISTIC ACID, non-polymer, 228.371 Da.

Natural source:

Natural source:   Common Name: virus   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11 and pET19

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Gag_polyprotein: GARASVASGGELDKWEKIRL RPGGKKQYKLKHIVWASREL ERFAVNPGLLETSEGCRQIL GQLQPSLQTGSEELRSLYNT IAVLYCVHQRIDVKDTKEAL DKIEEEQNKSKKKAQQAAAD TGNNSQVSQNY

Data sets:
Data typeCount
13C chemical shifts431
15N chemical shifts129
1H chemical shifts771

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HIV-1 L8A-myrMA1
2myr2

Entities:

Entity 1, HIV-1 L8A-myrMA 131 residues - Formula weight is not available

1   GLYALAARGALASERVALALASERGLYGLY
2   GLULEUASPLYSTRPGLULYSILEARGLEU
3   ARGPROGLYGLYLYSLYSGLNTYRLYSLEU
4   LYSHISILEVALTRPALASERARGGLULEU
5   GLUARGPHEALAVALASNPROGLYLEULEU
6   GLUTHRSERGLUGLYCYSARGGLNILELEU
7   GLYGLNLEUGLNPROSERLEUGLNTHRGLY
8   SERGLUGLULEUARGSERLEUTYRASNTHR
9   ILEALAVALLEUTYRCYSVALHISGLNARG
10   ILEASPVALLYSASPTHRLYSGLUALALEU
11   ASPLYSILEGLUGLUGLUGLNASNLYSSER
12   LYSLYSLYSALAGLNGLNALAALAALAASP
13   THRGLYASNASNSERGLNVALSERGLNASN
14   TYR

Entity 2, myr - C14 H28 O2 - 228.371 Da.

1   MYR

Samples:

sample_1: L8A, [U-100% 13C; U-100% 15N], 1 mM; Sodium phosphate 50 mM; NaCL 100 mM; DTT 5 mM; D2O 90 mM; H2O 10 mM

sample_2: L8A, [U-100% 15N], 1 mM; Sodium phosphate 50 mM; NaCL 100 mM; DTT 5 mM; D2O 90 mM; H2O 10 mM

sample_3: L8A 1 mM; Sodium phosphate 50 mM; NaCL 100 mM; DTT 5 mM; D2O 90 mM; H2O 10 mM

sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_3isotropicsample_conditions_1
3D_15N-separated_NOESYsample_2isotropicsample_conditions_1
3D_13C-separated_NOESYsample_1isotropicsample_conditions_1
4D_13C/15N-separated_NOESYsample_1isotropicsample_conditions_1
4D_13C-separated_NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - refinement

NMR spectrometers:

  • Bruker DRX 800 MHz
  • Bruker DMX 600 MHz

Related Database Links:

BMRB 15116 18715 18716
PDB
DBJ BAF34641 BAG48474
EMBL CBI61180 CBI61181 CBI61182 CBI61183 CBI61184
GB AAA44987 AAB00898 AAB60571 AAC28445 AAC29216
SP P12493 P12497
AlphaFold P12493 P12497

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks